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dc.contributor.authorHagopian, Kevork
dc.contributor.authorWeber, Kristina L.
dc.contributor.authorHwee, Darren T.
dc.contributor.authorVan Eenennaam, Alison L.
dc.contributor.authorLópez-Lluch, Guillermo
dc.contributor.authorVillalba, José Manuel
dc.contributor.authorBurón, Isabel
dc.contributor.authorNavas Lloret, Plácido
dc.contributor.authorGerman, Bruce
dc.contributor.authorWatkins, Steven
dc.contributor.authorChen, Yana
dc.contributor.authorWei, Alfreda
dc.contributor.authorMcDonald, Roger B.
dc.contributor.authorRamsey, Jon J.
dc.date.accessioned2013-12-23T09:28:35Z
dc.date.available2013-12-23T09:28:35Z
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/10396/11516
dc.description.abstractThe polyunsaturated nature of n-3 fatty acids makes them prone to oxidative damage. However, it is not clear if n-3 fatty acids are simply a passive site for oxidative attack or if they also modulate mitochondrial reactive oxygen species (ROS) production. The present study used fat-1 transgenic mice, that are capable of synthesizing n-3 fatty acids, to investigate the influence of increases in n-3 fatty acids and resultant decreases in the n-6:n-3 ratio on liver mitochondrial H2O2 production and electron transport chain (ETC) activity. There was an increase in n-3 fatty acids and a decrease in the n-6:n-3 ratio in liver mitochondria from the fat-1 compared to control mice. This change was largely due to alterations in the fatty acid composition of phosphatidylcholine and phosphatidylethanolamine, with only a small percentage of fatty acids in cardiolipin being altered in the fat-1 animals. The lipid changes in the fat-1 mice were associated with a decrease (p,0.05) in the activity of ETC complex I and increases (p,0.05) in the activities of complexes III and IV. Mitochondrial H2O2 production with either succinate or succinate/glutamate/malate substrates was also decreased (p,0.05) in the fat-1 mice. This change in H2O2 production was due to a decrease in ROS production from ETC complex I in the fat-1 animals. These results indicate that the fatty acid changes in fat-1 liver mitochondria may at least partially oppose oxidative stress by limiting ROS production from ETC complex I.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherPublic Libray of Science (PLOS)es_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourcePLoS ONE 5 (9) (2010)es_ES
dc.subjectn-3 fatty acidses_ES
dc.subjectoxidative damagees_ES
dc.titleComplex I-Associated Hydrogen Peroxide Production Is Decreased and Electron Transport Chain Enzyme Activities Are Altered in n-3 Enriched fat-1 Micees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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