Varietal Tracing of Virgin Olive Oils Based on Plastid DNA Variation Profiling
Autor
Pérez Jiménez, Margarita
Besnard, Guillaume
Dorado, G.
Hernández, Pilar
Editor
Public Library of ScienceFecha
2013Materia
Vegetable oilsOlive trees
Genetic loci
Haplotypes
Oils
Plastids
Polymerase chain reaction
Olives
METS:
Mostrar el registro METSPREMIS:
Mostrar el registro PREMISMetadatos
Mostrar el registro completo del ítemResumen
Olive oil traceability remains a challenge nowadays. DNA analysis is the preferred approach to an effective varietal
identification, without any environmental influence. Specifically, olive organelle genomics is the most promising approach
for setting up a suitable set of markers as they would not interfere with the pollinator variety DNA traces. Unfortunately,
plastid DNA (cpDNA) variation of the cultivated olive has been reported to be low. This feature could be a limitation for the
use of cpDNA polymorphisms in forensic analyses or oil traceability, but rare cpDNA haplotypes may be useful as they can
help to efficiently discriminate some varieties. Recently, the sequencing of olive plastid genomes has allowed the
generation of novel markers. In this study, the performance of cpDNA markers on olive oil matrices, and their applicability
on commercial Protected Designation of Origin (PDO) oils were assessed. By using a combination of nine plastid loci
(including multi-state microsatellites and short indels), it is possible to fingerprint six haplotypes (in 17 Spanish olive
varieties), which can discriminate high-value commercialized cultivars with PDO. In particular, a rare haplotype was detected
in genotypes used to produce a regional high-value commercial oil. We conclude that plastid haplotypes can help oil
traceability in commercial PDO oils and set up an experimental methodology suitable for organelle polymorphism detection
in the complex olive oil matrices.