Show simple item record

dc.contributor.authorValhondo, Isabel
dc.contributor.authorHassouneh, Fakhri
dc.contributor.authorLópez-Sejas, Nelson
dc.contributor.authorPera Rojas, Alejandra
dc.contributor.authorSánchez-Correa, Beatriz
dc.contributor.authorGuerrero, Beatriz
dc.contributor.authorBergua, Juan M.
dc.contributor.authorArcos, María José
dc.contributor.authorBañas, Helena
dc.contributor.authorCasas-Avilés, Ignacio
dc.contributor.authorSánchez García, Joaquín
dc.contributor.authorSerrano, Josefina
dc.contributor.authorMartín, Carmen
dc.contributor.authorDurán, Esther
dc.contributor.authorAlonso, Corona
dc.contributor.authorSolana, Rafael
dc.contributor.authorTarazona, Raquel
dc.date.accessioned2020-08-05T09:51:07Z
dc.date.available2020-08-05T09:51:07Z
dc.date.issued2020
dc.identifier.urihttp://hdl.handle.net/10396/20400
dc.description.abstractBackground: Acute myeloid leukemia (AML) remains a major clinical challenge due to poor overall survival, which is even more dramatic in elderly patients. TIGIT, an inhibitory receptor that interacts with CD155 and CD112 molecules, is considered as a checkpoint in T and NK cell activation. This receptor shares ligands with the co-stimulatory receptor DNAM-1 and with TACTILE. The aim of this work was to analyze the expression of DNAM-1, TIGIT and TACTILE in NK cells and T cell subsets in AML patients. Methods: We have studied 36 patients at the time of diagnosis of AML and 20 healthy volunteers. The expression of DNAM-1, TIGIT and TACTILE in NK cells and T cells, according to the expression of CD3 and CD56, was performed by flow cytometry. Results: NK cells, CD56− T cells and CD56+ T (NKT-like) cells from AML patients presented a reduced expression of DNAM-1 compared with healthy volunteers. An increased expression of TIGIT was observed in mainstream CD56− T cells. No differences were observed in the expression of TACTILE. Simplified presentation of incredibly complex evaluations (SPICE) analysis of the co-expression of DNAM-1, TIGIT and TACTILE showed an increase in NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE. Low percentages of DNAM-1−TIGIT+TACTILE+ NK cells and DNAM-1− TIGIT+TACTILE+ CD56− T cells were associated with a better survival of AML patients. Conclusions: The expression of DNAM-1 is reduced in NK cells and in CD4+ and CD8+ T cells from AML patients compared with those from healthy volunteers. An increased percentage of NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE is associated with patient survival, supporting the role of TIGIT as a novel candidate for checkpoint blockade.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceCancers 12(8), 2171 (2020)es_ES
dc.subjectAcute myeloid leukemiaes_ES
dc.subjectDNAM-1es_ES
dc.subjectNK cellses_ES
dc.subjectNKT-like cellses_ES
dc.subjectTACTILEes_ES
dc.subjectT cellses_ES
dc.subjectTIGITes_ES
dc.titleCharacterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemiaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/cancers12082171es_ES
dc.relation.projectIDGobierno de España. SAF2017-87538-Res_ES
dc.relation.projectIDInstituto de Salud Carlos III. PI16/01615es_ES
dc.relation.projectIDInstituto de Salud Carlos III. CP19/00008es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record