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Characterization and Analysis of Anthocyanin-Related Genes in Wild-Type Blueberry and the Pink-Fruited Mutant Cultivar ‘Pink Lemonade’: New Insights into Anthocyanin Biosynthesis
dc.contributor.author | Die, Jose V. | |
dc.contributor.author | Jones, Richard W. | |
dc.contributor.author | Ogden, Elizabeth L. | |
dc.contributor.author | Ehlenfeldt, Mark K. | |
dc.contributor.author | Rowland, Lisa J. | |
dc.date.accessioned | 2020-09-09T07:59:04Z | |
dc.date.available | 2020-09-09T07:59:04Z | |
dc.date.issued | 2020 | |
dc.identifier.uri | http://hdl.handle.net/10396/20426 | |
dc.description.abstract | Blueberries are one of the richest sources of antioxidants, such as anthocyanins, among fruits and vegetables. Anthocyanin mutants, like the pink-fruited cultivar ‘Pink Lemonade’, are valuable resources for investigating anthocyanin biosynthesis in blueberries. In this study, we examined expression of flavonoid pathway genes during fruit development in wild-type, blue-fruited blueberries using quantitative real-time PCR. Expression was also compared between wild-type and the pink-fruited ‘Pink Lemonade’. This revealed significantly lower expression in ‘Pink Lemonade’ than in wild-type of nearly all the structural genes examined suggesting that a transcriptional regulator of the pathway was affected. Hence, we compared expression of three known regulatory genes and found that the gene encoding the transcription factor MYB1 was expressed at a significantly lower level in ‘Pink Lemonade’ than in the wild-type. To validate the capacity of this MYB1 to regulate the transcription of anthocyanin genes in blueberries, a transient expression assay was conducted. Results indicated MYB1 overexpression enhanced anthocyanin production. Comparative sequence analysis between wild-type and mutant MYB1 variants found differences in highly conserved features suggesting a mechanistic explanation for the mutant phenotype. Collectively, the results presented here contribute to a better understanding of mechanisms regulating anthocyanin biosynthesis in Vaccinium. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.source | Agronomy 10(9), 1296 (2020) | es_ES |
dc.subject | Anthocyanins | es_ES |
dc.subject | Blueberry | es_ES |
dc.subject | Flavonoid | es_ES |
dc.subject | Fruit | es_ES |
dc.subject | MYB transcription factor | es_ES |
dc.subject | qPCR | es_ES |
dc.title | Characterization and Analysis of Anthocyanin-Related Genes in Wild-Type Blueberry and the Pink-Fruited Mutant Cultivar ‘Pink Lemonade’: New Insights into Anthocyanin Biosynthesis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/agronomy10091296 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |