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Elucidating cold acclimation pathway in blueberry by transcriptome profiling
(Elsevier, 2014)
A fundamental goal of cold acclimation research is to understand the mechanisms responsible for the increase in freezing tolerance in response to environmental cues. Changes in gene expression underlie some of the biochemical ...
Design and sampling plan optimization for RT-qPCR experiments in plants: a case study in blueberry
(Frontiers, 2016)
The qPCR assay has become a routine technology in plant biotechnology and agricultural research. It
is unlikely to be technically improved, but there are still challenges which center around minimizing
the variability ...
Superior Cross-Species Reference Genes: A Blueberry Case Study
(Public Library of Science (PLOS), 2013)
The advent of affordable Next Generation Sequencing technologies has had major impact on studies of many crop species, where access to genomic technologies and genome-scale data sets has been extremely limited until now. ...
RNA quality assessment: a view from plant qPCR studies
(Oxford University Press, 2012)
Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is probably the most common molecular technique used in transcriptome analyses today. The simplicity of the technology and associated protocols ...
Characterization of the 3’: 5’ ratio for reliable determination of RNA quality
(Elsevier, 2011)
Determination of RNA quality is a critical first step in obtaining meaningful gene expression data. The PCR-based 3’: 5’ assay is an RNA quality assessment tool. This assay is a simple, fast and low-cost method of selecting ...