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dc.contributor.authorCastillejo-Sánchez, María A.
dc.contributor.authorVillegas Fernández, Ángel María
dc.contributor.authorHernández-Lao, Tamara
dc.contributor.authorRubiales, Diego
dc.date.accessioned2021-11-08T09:21:19Z
dc.date.available2021-11-08T09:21:19Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/10396/22028
dc.description.abstractChocolate spot, which is caused by the necrotrophic fungus Botrytis fabae, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (Vicia faba). Although chemical control of this disease is an option, it has serious economic and environmental drawbacks that make resistant cultivars a more sensible choice. The molecular mechanisms behind the defense against B. fabae are poorly understood. In this work, we studied the leave proteome in two faba bean genotypes that respond differently to B. fabae in order to expand the available knowledge on such mechanisms. For this purpose, we used two-dimensional gel electrophoresis (2DE) in combination with Matrix-Assisted Laser Desorption/Ionization (MALDI-TOF/TOF). Univariate statistical analysis of the gels revealed 194 differential protein spots, 102 of which were identified by mass spectrometry. Most of the spots belonged to proteins in the energy and primary metabolism, degradation, redox or response to stress functional groups. The MS results were validated with assays of protease activity in gels. Overall, they suggest that the two genotypes may respond to B. fabae with a different PSII protein repair cycle mechanism in the chloroplast. The differences in resistance to B. fabae may be the result of a metabolic imbalance in the susceptible genotype and of a more efficient chloroplast detoxification system in the resistant genotype at the early stages of infection.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceAgronomy 11(11), 2247 (2021)es_ES
dc.subjectBotrytis fabaees_ES
dc.subjectFaba beanes_ES
dc.subjectResistancees_ES
dc.subjectProteomic analysises_ES
dc.subjectPhotosystem II repair cyclees_ES
dc.titlePhotosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to Botrytis fabae Infectiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.3390/agronomy11112247es_ES
dc.relation.projectIDGobierno de España. PCI2020-111974es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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