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dc.contributor.authorPrados de la Torre, Esther
dc.contributor.authorObando, Ignacio
dc.contributor.authorVidal, Marta
dc.contributor.authorFelipe, Beatriz de
dc.contributor.authorAguilar, Ruth
dc.contributor.authorIzquierdo, Luis
dc.contributor.authorCarolis, Carlo
dc.contributor.authorOlbrich, Peter
dc.contributor.authorCapilla-Miranda, Ana
dc.contributor.authorSerra, Pau
dc.contributor.authorSantamaria, Pere
dc.contributor.authorBlanco-Lobo, Pilar
dc.contributor.authorMoncunill, Gemma
dc.contributor.authorRodríguez-Ortega, Manuel J.
dc.contributor.authorDobaño, Carlota
dc.date.accessioned2022-09-15T11:04:38Z
dc.date.available2022-09-15T11:04:38Z
dc.date.issued2022
dc.identifier.urihttp://hdl.handle.net/10396/23897
dc.description.abstractSARS-CoV-2 infection has become a global health problem specially exacerbated with the continuous appearance of new variants. Healthcare workers (HCW) have been one of the most affected sectors. Children have also been affected, and although infection generally presents as a mild disease, some have developed the Pediatric Inflammatory Multisystem Syndrome Temporally Associated with SARS-CoV-2 (PIMS-TS). We recruited 190 adults (HCW and cohabitants, April to June 2020) and 57 children (April 2020 to September 2021), of whom 12 developed PIMS-TS, in a hospital-based study in Spain. Using an in-house Luminex assay previously validated, antibody levels were measured against different spike and nucleocapsid SARS-CoV-2 proteins, including the receptor-binding domain (RBD) of the Alpha, Beta, Gamma, and Delta variants of concern (VoC). Seropositivity rates obtained from children and adults, respectively, were: 49.1% and 11% for IgG, 45.6% and 5.8% for IgA, and 35.1% and 7.3% for IgM. Higher antibody levels were detected in children who developed PIMS-TS compared to those who did not. Using the COVID-19 IgM/IgA ELISA (Vircell, S.L.) kit, widely implemented in Spanish hospitals, a high number of false positives and lower seroprevalences compared with the Luminex estimates were found, indicating a significantly lower specificity and sensitivity. Comparison of antibody levels against RBD-Wuhan versus RBD-VoCs indicated that the strongest positive correlations for all three isotypes were with RBD-Alpha, while the lowest correlations were with RBD-Delta for IgG, RBD-Gamma for IgM, and RBD-Beta for IgA. This study highlights the differences in antibody levels between groups with different demographic and clinical characteristics, as well as reporting the IgG, IgM, and IgA response to RBD VoC circulating at the study period.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceViruses 14(9), 2039 (2022)es_ES
dc.subjectSARS-CoV-2es_ES
dc.subjectAntibodyes_ES
dc.subjectAntigenes_ES
dc.subjectCOVID-19es_ES
dc.subjectSeropositivityes_ES
dc.subjectCohortes_ES
dc.subjectChildrenes_ES
dc.subjectPIMS-TSes_ES
dc.subjectHealthcare workerses_ES
dc.titleSARS-CoV-2 Seroprevalence Study in Pediatric Patients and Health Care Workers Using Multiplex Antibody Immunoassayses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.3390/v14092039es_ES
dc.relation.projectIDJunta de Andalucía. CV20-204195es_ES
dc.relation.projectIDJunta de Andalucía. PP2021 SUBMOD2.1es_ES
dc.relation.projectIDJunta de Andalucía. 1380508-Res_ES
dc.relation.projectIDGobierno de España. CEX2018-000806-Ses_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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