Antimicrobial activity of Lactiplantibacillus plantarum against shiga toxin-producing
Autor
Ruiz, María Julia
García, Mauro Daniel
Medina Canalejo, Luis M.
Krüger, Nora
Padola, Nora L.
Etcheverría, Analía Inés
Editor
OxfordFecha
2023Materia
Lactic acid bacteriaPlantaricin
Shiga toxin-producing E. coli
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Aims
The aim of the present work was to characterize the Lactiplantibacillus sp. LP5 strain, isolated from pork production, and identify bacteriocin-like inhibitory substances produced by this strain.
Methods and Results
In this study, LP5 was identified by species-specific PCR and 16S rRNA sequencing. Additionally, bacterial growth kinetics, antimicrobial activity, the detection of genes related to plantaricin production, and the genetic expression of plantaricins were determined. Lactiplantibacillus sp. LP5 was identified as Lactiplantibacillus plantarum. The well-diffusion test using cell-free supernatants (CFS), neutralized CFS, CFS treated with catalase, and CFS treated with proteinase K showed that inhibitory effects on a Shiga toxin-producing Escherichia coli (STEC) strain were produced by bacteriocins. The PCR technique allowed the detection of genes encoding E/F plantaricins, as well as J/K and whole genome sequencing, and bacteriocin mining analysis allowed us to confirm the presence of these plantaricins.
Conclusions
We can conclude that the inhibitory effect of L. plantarum LP5 isolated from pigs against the STEC EDL933 strain could be associated with the bacteriocins production and represents a potential use as a probiotic strain.
Descripción
Embargado hasta 30/09/2024