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Characterization of the 3’: 5’ ratio for reliable determination of RNA quality
dc.contributor.author | Die, Jose V. | |
dc.contributor.author | Obrero, Ángeles | |
dc.contributor.author | González-Verdejo, Clara I. | |
dc.contributor.author | Román, Belén | |
dc.date.accessioned | 2024-02-05T16:33:53Z | |
dc.date.available | 2024-02-05T16:33:53Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.uri | http://hdl.handle.net/10396/27133 | |
dc.description.abstract | Determination of RNA quality is a critical first step in obtaining meaningful gene expression data. The PCR-based 3’: 5’ assay is an RNA quality assessment tool. This assay is a simple, fast and low-cost method of selecting samples for further analysis. However, the practical application is unexploited primarily because of the absence of a experimental threshold. We show that, by anchoring the 5’assay a specific distance from the 3’ end of the sequence and by spacing the 3’ at a distance of a number of nucleotides, a cutoff determines whether a sample is suitable for downstream quantification studies. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Die, J. V., Obrero, Á., González-Verdejo, C. I., & Román, B. (2011). Characterization of the 3′:5′ ratio for reliable determination of RNA quality. Analytical Biochemistry, 419(2), 336-338. https://doi.org/10.1016/j.ab.2011.08.012 | es_ES |
dc.subject | 3':5' ratio | es_ES |
dc.subject | qPCR | es_ES |
dc.subject | RNA quality | es_ES |
dc.title | Characterization of the 3’: 5’ ratio for reliable determination of RNA quality | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.ab.2011.08.012 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |