An approach for estimating the maximum specific growth rate of Gluconobacter japonicus in strawberry purée without cell concentration data
Autor
Cañete Rodríguez, Ana María
Santos-Dueñas, Inés María
Jiménez Hornero, Jorge
Torija-Martínez, María J.
Mas, Albert
García García, Isidoro
Editor
ElsevierFecha
2016Materia
Maximum specific growth rateGluconobacter japonicus CECT 8443
Growth kinetics
Biokinetics
Biotransformations
Batch processing
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The estimation of the maximum specific growth rate ( max) for non-readily culturable bacteria, growing on complex media containing suspended solids, is a difficult task considering the important problems in obtaining reliable measures of cell concentration. An example of this situation can be a culture of Gluconobacter japonicus growing in strawberry purée for producing gluconic acid. Based on the dependency between energy requirements of the genus Gluconobacter and substrate uptake as well as its constant relationship between gluconic acid production and total substrate uptake, the total substrate concentration
profile during the exponential growth phase could be used for estimating max without cell concentration measures. In this case, the high selectivity of the strain for glucose in comparison to fructose resulted in no fructose consumption during the batch; so, just using the glucose concentrations data during the exponential phase allow us to obtain an estimation of mumax. Additionally, a rough estimation of the apparent and stoichiometric yields of cell on glucose is also possible.