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dc.contributor.authorBottrel, M.
dc.contributor.authorHidalgo, Manuel
dc.contributor.authorMogas, T.
dc.contributor.authorPereira, Blasa
dc.contributor.authorOrtiz, I.
dc.contributor.authorDíaz-Jiménez, María
dc.contributor.authorConsuegra, César
dc.contributor.authorMorató, R.
dc.contributor.authorDorado, Jesús
dc.date.accessioned2024-06-03T10:08:16Z
dc.date.available2024-06-03T10:08:16Z
dc.date.issued2020
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/10396/28439
dc.description.abstractThe objective of this study was to compare the effects of two warming protocols (three-step vs. one-step dilution) on embryo quality, post-warming embryo survival and embryo cell viability of donkey embryos vitrified by the Cryotop method. Twenty, Day 7–8, grade 1–2 donkey embryos were measured, morphologically evaluated and vitrified using the Cryotop technique. Embryos were then randomly warmed using two different warming procedures: (i) W3 (three-step dilution; n = 11): embryos were warmed in 1 M, 0.5 M and 0 M sucrose, and (ii) W1/0.5 (one-step dilution; n = 9): embryos were warmed directly in 0.5 M sucrose. After 3 and 24 h of warming, the embryos were measured and evaluated for their morphology, developmental stage and viability (Propidium Iodide-Hoechst 33,342 dyes). Although both treatments decreased embryo quality after warming (P < 0.05), no significant differences (P > 0.05) were observed between protocols in terms of post-warming embryo quality, diameter and embryo survival. Greater percentages of dead cells (P < 0.001) were observed when embryos were warmed directly in 0.5 M sucrose (one-step dilution) when compared to the three-step protocol. The percentage of ruptured embryos was 27.3% and 0% in W3 and W1/0.5 protocols (P = 0.0893), respectively. In conclusion, warming Cryotop-vitrified donkey embryos directly in 0.5 M sucrose had no negative effects on embryo quality and post-warming embryo survival. Moreover, one-step protocol may help to prevent rupture when donkey embryos warmed directly in 0.5 M sucrose. These results observed in vitro must be verified by embryo transfer.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceBottrel, M., Hidalgo, M., Mogas, T., Pereira, B., Ortiz, I., Díaz-Jiménez, M., Consuegra, C., Morató, R., & Dorado, J. (2020). One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos. Theriogenology, 152, 47-52. https://doi.org/10.1016/j.theriogenology.2020.04.026es_ES
dc.subjectDonkey embryoes_ES
dc.subjectCryotop vitrificationes_ES
dc.subjectSucrose dilutiones_ES
dc.subjectOne-step warminges_ES
dc.subjectStepwise warminges_ES
dc.titleOne-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryoses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.theriogenology.2020.04.026es_ES
dc.relation.projectIDGobierno de España. AGL2013-42726-Res_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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