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dc.contributor.authorBottrel, M.
dc.contributor.authorMogas, T.
dc.contributor.authorPereira, Blasa
dc.contributor.authorOrtiz, I.
dc.contributor.authorDíaz-Jiménez, María
dc.contributor.authorConsuegra, César
dc.contributor.authorHidalgo, Manuel
dc.contributor.authorMorató, R.
dc.contributor.authorDorado, Jesús
dc.date.accessioned2024-06-03T11:59:17Z
dc.date.available2024-06-03T11:59:17Z
dc.date.issued2020
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/10396/28444
dc.description.abstractMany domestic donkey breeds are at risk of extinction, there is a critical urgency for genome resource banking. In the present study, we examined whether the use of Ficoll 70 added to the vitrification medium containing ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose improves the cryotolerance of donkey in vivo derived embryos. Day 7–8, grade 1–2 donkey embryos were measured and morphologically evaluated and then vitrified-warmed using the Cryotop technique. Before vitrification, embryos were randomly distributed into two groups: (i) VS1 (n = 14): vitrified using 15% EG + 15% DMSO + 0.5 M sucrose; and (ii) VS2 (n = 10): vitrified in the same medium supplemented also with 18% of Ficoll 70. After 24 h of warming, the embryos were measured and evaluated for their morphology, development and viability (Propidium Iodide-Hoechst 33342 dyes). Post-warming survival was numerically higher but not significantly different (P > 0.05) when embryos were vitrified in VS2 (70%) compared to VS1 (57.1%). Embryo rupture was only observed in the VS1 group (21.4%, 3/14). Higher embryo diameter was observed in all groups after 24 h culture (P < 0.05). No significant differences (P > 0.05) were observed among treatments in terms of percentages of cell death. These results demonstrate that the addition of Ficoll 70 to the vitrification medium is not a pre-requisite for successful vitrification of donkey embryos. However, its addition seems to enhance some of the post-warming embryo quality characteristics. Since no statistically significant evidence was found, further studies should be conducted in order to confirm our findings.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceBottrel, M., Mogas, T., Pereira, B., Ortiz, I., Díaz-Jiménez, M., Consuegra, C., Hidalgo, M., Morató, R., & Dorado, J. (2020). The cryoprotective effect of Ficoll 70 on the post-warming survival and quality of Cryotop-vitrified donkey embryos. Theriogenology, 148, 180-185. https://doi.org/10.1016/j.theriogenology.2019.11.013es_ES
dc.subjectDonkeyses_ES
dc.subjectEmbryoes_ES
dc.subjectMacromoleculees_ES
dc.subjectVitrificationes_ES
dc.subjectViabilityes_ES
dc.titleThe cryoprotective effect of Ficoll 70 on the post-warming survival and quality of Cryotop-vitrified donkey embryoses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.theriogenology.2019.11.013es_ES
dc.relation.projectIDGobierno de España. AGL2013-42726-Res_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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