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dc.contributor.authorHerencia, Carmen
dc.contributor.authorMartínez-Moreno, Julio M.
dc.contributor.authorHerrera, Concepción
dc.contributor.authorCorrales, Fernando J.
dc.contributor.authorSantiago-Mora, Raquel
dc.contributor.authorEspejo, Isabel
dc.contributor.authorBarcos, Montserrat
dc.contributor.authorAlmadén Peña, Yolanda
dc.contributor.authorMata, Manuel de la
dc.contributor.authorRodríguez-Ariza, Antonio
dc.contributor.authorMuñoz-Castañeda, Juan R.
dc.date.accessioned2013-12-23T09:59:37Z
dc.date.available2013-12-23T09:59:37Z
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10396/11520
dc.description.abstractWnt/b-catenin pathway controls biochemical processes related to cell differentiation. In committed cells the alteration of this pathway has been associated with tumors as hepatocellular carcinoma or hepatoblastoma. The present study evaluated the role of Wnt/b-catenin activation during human mesenchymal stem cells differentiation into hepatocytes. The differentiation to hepatocytes was achieved by the addition of two different conditioned media. In one of them, b-catenin nuclear translocation, up-regulation of genes related to the Wnt/b-catenin pathway, such as Lrp5 and Fzd3, as well as the oncogenes c-myc and p53 were observed. While in the other protocol there was a Wnt/b-catenin inactivation. Hepatocytes with nuclear translocation of b-catenin also had abnormal cellular proliferation, and expressed membrane proteins involved in hepatocellular carcinoma, metastatic behavior and cancer stem cells. Further, these cells had also increased auto-renewal capability as shown in spheroids formation assay. Comparison of both differentiation protocols by 2D-DIGE proteomic analysis revealed differential expression of 11 proteins with altered expression in hepatocellular carcinoma. Cathepsin B and D, adenine phosphoribosyltransferase, triosephosphate isomerase, inorganic pyrophosphatase, peptidyl-prolyl cis-trans isomerase A or lactate dehydrogenase b-chain were up-regulated only with the protocol associated with Wnt signaling activation while other proteins involved in tumor suppression, such as transgelin or tropomyosin b-chain were downregulated in this protocol. In conclusion, our results suggest that activation of the Wnt/b-catenin pathway during human mesenchymal stem cells differentiation into hepatocytes is associated with a tumoral phenotypees_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherPublic Libray of Science (PLOS)es_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourcePLoS ONE 7 (4) (2012)es_ES
dc.subjectHepatocellular carcinomaes_ES
dc.subjectHepatoblastomaes_ES
dc.subjectWnt/b-catenines_ES
dc.titleNuclear Translocation of b-Catenin during Mesenchymal Stem Cells Differentiation into Hepatocytes Is Associated with a Tumoral Phenotypees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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