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dc.contributor.authorPulido, Marina R.
dc.contributor.authorDíaz-Ruiz, Alberto
dc.contributor.authorJiménez-Gómez, Yolanda
dc.contributor.authorGarcía-Navarro, Socorro
dc.contributor.authorGracia-Navarro, F.
dc.contributor.authorFrühbeck, Gema
dc.contributor.authorVázquez Martínez, Rafael
dc.contributor.authorTinahones, Francisco J.
dc.contributor.authorMalagón, María M.
dc.contributor.authorLópez-Miranda, José
dc.date.accessioned2013-12-23T10:20:21Z
dc.date.available2013-12-23T10:20:21Z
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10396/11522
dc.description.abstractLipid droplets (LDs) are organelles that coordinate lipid storage and mobilization, both processes being especially important in cells specialized in managing fat, the adipocytes. Proteomic analyses of LDs have consistently identified the small GTPase Rab18 as a component of the LD coat. However, the specific contribution of Rab18 to adipocyte function remains to be elucidated. Herein, we have analyzed Rab18 expression, intracellular localization and function in relation to the metabolic status of adipocytes. We show that Rab18 production increases during adipogenic differentiation of 3T3-L1 cells. In addition, our data show that insulin induces, via phosphatidylinositol 3-kinase (PI3K), the recruitment of Rab18 to the surface of LDs. Furthermore, Rab18 overexpression increased basal lipogenesis and Rab18 silencing impaired the lipogenic response to insulin, thereby suggesting that this GTPase promotes fat accumulation in adipocytes. On the other hand, studies of the badrenergic receptor agonist isoproterenol confirmed and extended previous evidence for the participation of Rab18 in lipolysis. Together, our data support the view that Rab18 is a common mediator of lipolysis and lipogenesis and suggests that the endoplasmic reticulum (ER) is the link that enables Rab18 action on these two processes. Finally, we describe, for the first time, the presence of Rab18 in human adipose tissue, wherein the expression of this GTPase exhibits sex- and depot-specific differences and is correlated to obesity. Taken together, these findings indicate that Rab18 is involved in insulin-mediated lipogenesis, as well as in b-adrenergic-induced lipolysis, likely facilitating interaction of LDs with ER membranes and the exchange of lipids between these compartments. A role for Rab18 in the regulation of adipocyte biology under both normal and pathological conditions is proposed.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherPublic Libray of Science (PLOS)es_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourcePLoS ONE 6 (7) (2011)es_ES
dc.subjectRab 18es_ES
dc.subjectLipid dropletses_ES
dc.subjectLipogenesises_ES
dc.titleRab18 Dynamics in Adipocytes in Relation to Lipogenesis, Lipolysis and Obesityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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