dc.contributor.author | Ruiz, Antonio | |
dc.contributor.author | Molina, José Manuel | |
dc.contributor.author | González, Jorge | |
dc.contributor.author | Martínez-Moreno, Francisco Javier | |
dc.contributor.author | Gutiérrez, Pedro Nolasco | |
dc.contributor.author | Martínez-Moreno, Álvaro | |
dc.date.accessioned | 2017-01-17T12:08:03Z | |
dc.date.available | 2017-01-17T12:08:03Z | |
dc.date.issued | 2003 | |
dc.identifier.uri | http://hdl.handle.net/10396/14307 | |
dc.description.abstract | The use of cysteine proteinases from Fasciola hepatica adult flukes for the
serodiagnosis of caprine fasciolosis by means of an indirect ELISA test was studied. Two
proteolytic fractions from adult fluke homogenates, with apparent molecular weights of 28 and
34 kDa (P28 and P34 respectively), were characterised as cysteine proteinases using azocasein
assays and gelatin gel analysis. Both P28 and P34 fractions were electroluted and used as antigens
in two different indirect ELISA tests. Serum IgG levels against P28 and P34 in goats given an
experimental primary infection with 200 metacercariae or in goats given two experimental
infections with 200 metacercariae were determined and compared with those observed in an
uninfected control group. ELISA tests using both cysteine proteases showed a rapid and consistent
detection of specific IgG in all experimentally infected goats. The IgG response to P28 was the first
to be detected as early as 2–3 weeks post-infection and remained elevated throughout the
experiment. The response to P34 was detected later (4–6 wpi) and disappeared in some animals at
18 wpi, while flukes were still present in the bile ducts. No significant differences were observed
between the anti-P28 and anti-P34 IgG responses between animals receiving a primary or a
challenge infection. The results of our study, although preliminary, are promising since the P28
ELISA described here may be a reliable method for the immunodiagnosis of F. hepatica infection
in goats. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | EDP sciences | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Veterinary Research 34, 435-443 (2003) | es_ES |
dc.subject | Fasciola hepatica | es_ES |
dc.subject | Cysteine proteinases | es_ES |
dc.subject | IgG | es_ES |
dc.subject | ELISA | es_ES |
dc.subject | Goat | es_ES |
dc.title | Humoral response (IgG) of goats experimentally infected with Fasciola hepatica against cysteine proteinases of adult fluke | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |