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dc.contributor.authorTomás, Diana
dc.contributor.authorDias, Ana Luisa
dc.contributor.authorSilva, Manuela
dc.contributor.authorOliveira, Hugo R.
dc.contributor.authorSuso, María José
dc.contributor.authorViegas, Wanda
dc.contributor.authorVeloso, María Manuela
dc.date.accessioned2017-11-09T08:24:35Z
dc.date.available2017-11-09T08:24:35Z
dc.date.issued2016
dc.identifier.urihttp://hdl.handle.net/10396/15380
dc.description.abstractFaba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceDiversity 8(2), 8 (2016)es_ES
dc.subjectFaba bean locally adapted populationses_ES
dc.subjectGenomic diversityes_ES
dc.subjectIRAPes_ES
dc.titleGenetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markerses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/d8020008es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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