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dc.contributor.authorGonzález-Ballester, David
dc.contributor.authorSanz-Luque, Emanuel
dc.contributor.authorGalván Cejudo, Aurora
dc.contributor.authorFernández Reyes, Emilio
dc.contributor.authorMontaigu, Amaury de
dc.date.accessioned2018-05-02T07:45:18Z
dc.date.available2018-05-02T07:45:18Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/10396/16550
dc.description.abstractNitrogen assimilation and metabolism are essential processes for all living organisms, yet there is still much to be learnt on how they are regulated. The use of Chlamydomonas reinhardtii as a model system has been instrumental not only in identifying conserved regulation mechanisms that control the nitrogen assimilation pathway, but also in understanding how the intracellular nitrogen status regulates metabolic processes of industrial interest such as the synthesis of biolipids. While the genetic regulators that control the nitrogen pathway are successfully being unravelled, other layers of regulation have received less attention. Amino acids, for example, regulate nitrogen assimilation in certain organisms, but their role in Chlamydomonas has not thoroughly been explored. Previous results had suggested that arginine might repress key genes of the nitrogen assimilation pathway by acting within the ammonium negative signalling cascade, upstream of the nitric oxide (NO) inducible guanylate cyclase CYG56. We tested this hypothesis with a combination of genetic and chemical approaches. Antagonising the effects of arginine with an arginine biosynthesis mutant or with two chemical analogues released gene expression from ammonium mediated repression. The cyg56 and related non1 mutants, which are partially insensitive to ammonium repression, were also partially insensitive to repression by arginine. Finally, we show that the addition of arginine to the medium leads to an increase in intracellular NO. Our data reveal that arginine acts as a negative signal for the assimilation of nitrogen within the ammonium-CYG56 negative signalling cascade, and provide a connection between amino acid metabolism and nitrogen assimilation in microalgae.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourcePloS ONE 13 (4): e0196167 (2018)es_ES
dc.subjectNitrogen metabolismes_ES
dc.subjectNitrogen assimilationes_ES
dc.subjectChlamydomonases_ES
dc.subjectCYG56es_ES
dc.titleArginine is a component of the ammonium- CYG56 signalling cascade that represses genes of the nitrogen assimilation pathway in Chlamydomonas reinhardtiies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0196167es_ES
dc.relation.projectIDGobierno de España. BFU2015- 70649-Pes_ES
dc.relation.projectIDJunta de Andalucía. BIO-502es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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