Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA: In Vivo and In Vitro Studies
Autor
Tasset, Inmaculada
Fernández Bedmar, Zahira Noemí
Lozano Baena, María Dolores
Campos-Sánchez, J.
Haro Bailón, Antonio de
Muñoz Serrano, Andrés
Alonso Moraga, Ángeles
Editor
Public Library of ScienceFecha
2013Materia
Borage seed oilDegenerative diseases
Gamma linoleic acid
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Borage (Borago officinalis L.) seed oil has been used as a treatment for various degenerative diseases. Many useful properties
of this oil are attributed to its high gamma linolenic acid content (GLA, 18:3 v-6). The purpose of this study was to
demonstrate the safety and suitability of the use of borage seed oil, along with one of its active components, GLA, with
respect to DNA integrity, and to establish possible in vivo toxic and in vitro cytotoxic effects. In order to measure these
properties, five types of assays were carried out: toxicity, genotoxicity, antigenotoxicity, cytotoxicity (using the
promyelocytic leukaemia HL60 cell line), and life span (in vivo analysis using the Drosophila model). Results showed that
i) Borage seed oil is not toxic to D. melanogaster at physiological concentrations below 125 ml/ml and the studies on GLA
indicated non-toxicity at the lowest concentration analyzed ii) Borage seed oil and GLA are DNA safe (non-genotoxic) and
antimutagenic compared to hydrogen peroxide, thereby confirming its antioxidant capacity; iii) Borage seed oil and GLA
exhibited cytotoxic activity in low doses (IC50 of 1 ml/ml and 0.087 mM, respectively) iv) Low doses of borage seed oil
(0.19%) increased the health span of D. melanogaster; and v) GLA significantly decreased the life span of D.
melanogaster. Based on the antimutagenic and cytotoxic effects along with the ability to increase the health span, we
propose supplementation with borage seed oil rather than GLA, because it protects DNA by modulating oxidative genetic
damage in D. melanogaster, increases the health span and exerts cytotoxic activity towards promyelocytic HL60 cells.