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dc.contributor.authorLópez-Grueso, María José
dc.contributor.authorTarradas Valero, Rosa María
dc.contributor.authorCarmona Hidalgo, Beatriz
dc.contributor.authorLagal Ruiz, Daniel
dc.contributor.authorPeinado, José
dc.contributor.authorMcDonagh, B.
dc.contributor.authorRequejo Aguilar, Raquel
dc.contributor.authorBárcena, J.A.
dc.contributor.authorPadilla C., Alicia
dc.date.accessioned2019-10-23T11:37:56Z
dc.date.available2019-10-23T11:37:56Z
dc.date.issued2019
dc.identifier.urihttp://hdl.handle.net/10396/19043
dc.description.abstractPeroxiredoxin 6 (Prdx6) is the only member of 1-Cys subfamily of peroxiredoxins in human cells. It is the only Prdx acting on phospholipid hydroperoxides possessing two additional sites with phospholipase A2 (PLA2) and lysophosphatidylcholine-acyl transferase (LPCAT) activities. There are contrasting reports on the roles and mechanisms of multifunctional Prdx6 in several pathologies and on its sensitivity to, and influence on, the redox environment. We have down-regulated Prdx6 with specific siRNA in hepatoblastoma HepG2 cells to study its role in cell proliferation, redox homeostasis, and metabolic programming. Cell proliferation and cell number decreased while cell volume increased; import of glucose and nucleotide biosynthesis also diminished while polyamines, phospholipids, and most glycolipids increased. A proteomic quantitative analysis suggested changes in membrane arrangement and vesicle trafficking as well as redox changes in enzymes of carbon and glutathione metabolism, pentose-phosphate pathway, citrate cycle, fatty acid metabolism, biosynthesis of aminoacids, and Glycolysis/Gluconeogenesis. Specific redox changes in Hexokinase-2 (HK2), Prdx6, intracellular chloride ion channel-1 (CLIC1), PEP-carboxykinase-2 (PCK2), and 3-phosphoglycerate dehydrogenase (PHGDH) are compatible with the metabolic remodeling toward a predominant gluconeogenic flow from aminoacids with diversion at 3-phospohglycerate toward serine and other biosynthetic pathways thereon and with cell cycle arrest at G1/S transition.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceAntioxidants 8(11), 505 (2019)es_ES
dc.subjectPeroxiredoxines_ES
dc.subjectThiol redox regulationes_ES
dc.subjectRedox proteomees_ES
dc.subjectRedox homeostasises_ES
dc.subjectLipid metabolismes_ES
dc.subjectCell cyclees_ES
dc.titlePeroxiredoxin 6 Down-Regulation Induces Metabolic Remodeling and Cell Cycle Arrest in HepG2 Cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/antiox8110505es_ES
dc.relation.projectIDGobierno de España. BFU2016-80006-Pes_ES
dc.relation.projectIDJunta de Andalucía. BIO-0216es_ES
dc.relation.projectIDJunta de Andalucía. EJ17-BIO216es_ES
dc.relation.projectIDJunta de Andalucía. EJI-17-BIO216es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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