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dc.contributor.authorGarcía, Verónica
dc.contributor.authorCastro, Patricia
dc.contributor.authorTurbet-Delof, Michel
dc.contributor.authorGil, Juan
dc.contributor.authorMoreno, Roberto
dc.date.accessioned2021-07-19T10:32:39Z
dc.date.available2021-07-19T10:32:39Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/10396/21489
dc.description.abstractThe development of new asparagus (Asparagus officinalis L.) cultivars has been hindered by the lack of genetic diversity in the crop. The current study aims at generating novel plant material to widen the genetic base of the crop by using CWR (crop wild relatives). We have developed a hexaploid population of about 1000 plants with introgressions of four polyploid CWR species (A. maritimus, A. pseudoscaber, A. brachyphyllus and A. macrorrhizus). The population is the second generation from a first backcrossing using the landrace ‘Morado de Huétor’ as recurrent parent and was obtained in open pollination. The diversity of the population was analyzed by six EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat) markers. A random sampling of 60 plants was used to estimate the genetic variability. The results were compared with data previously obtained from the parental collection, the landrace ‘Morado de Huétor’ and diploid current asparagus cultivars. The average PICm (polymorphic information content) value of the new population and ‘Morado de Huétor’ were similar (>0.8) and higher than the value observed in the diploid cultivars (0.61). At least 22.2% of the alleles detected in the hexaploid population were specific from the CWR species used in this study. Principal coordinate analyses (PCoA) revealed a clear genetic diversity differentiation between populations. The population of 1000 plants was evaluated for agronomic traits (earliness, stalk number, branching height and stalk thickness) for two years and we selected 80 plants to develop a breeding base population. Variation was significant for all traits (P<0.001). These 80 plants were also analyzed with the six EST-SSR markers and they conserve the variability present in the starting population. The results show an increase in the genetic variability and offer a new opportunity for asparagus improvement.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceScientia Horticulturae 287, 11027 (2021)es_ES
dc.subjectPolyploidyes_ES
dc.subjectGenetic resourceses_ES
dc.subjectLandracees_ES
dc.subjectCurrent asparagus cultivarses_ES
dc.subjectEST-SSR markerses_ES
dc.subjectAsparagus improvementes_ES
dc.titleDevelopment and diversity analysis of an hexaploid pre-breeding asparagus population with introgressions from wild relative specieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.scienta.2021.110273es_ES
dc.relation.projectIDGobierno de España. AGL2014–57575Res_ES
dc.relation.projectIDGobierno de España. RFP2015–00,005–00–00es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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