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Genetic Variability Assessment of a Diploid Pre-Breeding Asparagus Population Developed Using the Tetraploid Landrace ‘Morado de Huétor’
dc.contributor.author | García, Verónica | |
dc.contributor.author | Castro, Patricia | |
dc.contributor.author | Millán, Teresa | |
dc.contributor.author | Gil, Juan | |
dc.contributor.author | Moreno, Roberto | |
dc.date.accessioned | 2022-09-22T08:41:23Z | |
dc.date.available | 2022-09-22T08:41:23Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | http://hdl.handle.net/10396/23956 | |
dc.description.abstract | Different studies have reported a narrow genetic base for garden asparagus (Asparagus officinalis L.) due to its common origin, a diploid population (‘Purple Dutch’). The present study focused on the development of new diploid plant material that may be useful to widen the genetic base of the crop by using a tetraploid landrace ‘Morado de Huétor’ (A. officinalis × A. maritimus). With this purpose, a diploid pre-breeding population (n = 1000) carrying introgressions of ‘Morado de Huétor’ has been obtained. This new population derived from crosses under open pollination of a parental collection (n = 77) that was developed in a previous study. The parental collection derived from the first backcrossing using different diploid cultivated plants as a recurrent parent and ‘Morado de Huétor’ as a donor. The genetic diversity of the pre-breeding population was assessed using a set of EST-SSR markers (AG7, AG8, TC1, TC3, TC7, TC9) in a collection of plants (n = 57), which was randomly sampled in the pre-breeding population. The results were compared to previous data obtained from the parental collection, a set of current diploid asparagus cultivars and the landrace ‘Morado de Huétor’. The average of PICm (Polymorphic Information Content) values obtained in the pre-breeding population (0.75) resulted higher than the value obtained in the diploid cultivars (0.63) but lower than in ‘Morado de Huétor’ (0.83). Twenty-two alleles (52.4%) detected in the new diploid population were specific from ‘Morado de Huétor’. Principal Coordinate Analyses (PCoA) revealed that the new population had a genetic diversity distribution different from the current cultivars. This new population was also evaluated for different morpho-agronomic traits (earliness, stalk number, branching height and stalk thickness) for two years. Significant differences among plants (p < 0.001) were found for these five traits and, therefore, a genotype variation is suggested. As a result, 71 plants were selected to develop a breeding base population. The genetic variability of those selected plants was also analyzed and similar genetic variability to the pre-breeding population was obtained. The results obtained in this study show that this new population could be used to enlarge the genetic base of the current diploid asparagus cultivars | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Horticulturae, 8(10), 859 (2022) | es_ES |
dc.subject | Asparagus improvement | es_ES |
dc.subject | Pre-breeding | es_ES |
dc.subject | Breeding base | es_ES |
dc.subject | Genetic resources | es_ES |
dc.subject | Landrace | es_ES |
dc.subject | Genetic variability | es_ES |
dc.subject | EST-SSR markers | es_ES |
dc.title | Genetic Variability Assessment of a Diploid Pre-Breeding Asparagus Population Developed Using the Tetraploid Landrace ‘Morado de Huétor’ | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/horticulturae8100859 | es_ES |
dc.relation.projectID | Gobierno de España. GL2014–57575-R | |
dc.relation.projectID | Gobierno de España. PID2019-106991RB-10 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |