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dc.contributor.authorGuzmán, Carlos
dc.contributor.authorCaballero, Leonor
dc.contributor.authorÁlvarez, Juan B.
dc.date.accessioned2024-01-30T11:52:48Z
dc.date.available2024-01-30T11:52:48Z
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/10396/26833
dc.description.abstractEmmer wheat is a neglected crop that could be used in the breeding of modern durum wheat for quality, one important aspect of which is the starch composition that is related to the waxy proteins. A collection of 87 accessions of Spanish emmer wheat was analysed for waxy protein composition by SDS– PAGE. No polymorphism was found for the Wx-A1 gene. However, for the Wx-B1 gene, three alleles were detected, two of them new. The whole gene sequence of these alleles was amplified by PCR in three fragments, which were digested with several endonucleases to determine internal differences in the sequence. These variants were also compared with the Wx alleles present in durum wheat. Differences in size and restriction sites were detected. DNA sequence analysis confirmed that the alleles found in emmer wheat are different from those in durum wheat. The first data suggested that these alleles showed a different influence on the amylose content of these lines. The variation found could be used to enlarge the gene pool of durum and emmer wheat, and design new materials with different amylose content.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceGuzmán, C., Caballero, L., & Álvarez, J. B. (2010). Molecular characterisation of the WX-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat. Molecular Breeding, 28(3), 403-411. https://doi.org/10.1007/s11032-010-9493-2es_ES
dc.subjectAmylose contentes_ES
dc.subjectEmmer wheates_ES
dc.subjectGenetic polymorphismes_ES
dc.subjectWaxy proteinses_ES
dc.titleMolecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheates_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1007/s11032-010-9493-2es_ES
dc.relation.projectIDGobierno de España. AGL2007-65685-C02-02es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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