Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

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Author
Gutiérrez-Gálvez, Laura
Del Caño-Ochoa, Rafael
Menéndez-Luque, Iris
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, Mónica
Pineda, Teresa
Pariente, Félix
García-Mendiola, Tania
Lorenzo, Encarnación
Publisher
Elsevier
Date
2022
Subject
AuNMs
DNA biosensor
SARS-CoV-2
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Abstract
This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARSCoV- 2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)3]2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)3]2+/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.
URI
http://hdl.handle.net/10396/27084
Fuente
Gutiérrez-Gálvez, L., Del Caño, R., Menéndez-Luque, I., García-Nieto, D., Rodríguez-Peña, M., Luna, M. V., Pineda, T., Pariente, F., García‐Mendiola, T., & Lorenzo, E. (2022b). Electrochemiluminescent nanostructured DNA biosensor for SARS-COV-2 detection. Talanta, 240, 123203. https://doi.org/10.1016/j.talanta.2021.123203
Versión del Editor
https://doi.org/10.1016/j.talanta.2021.123203
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