FaMYB123 interacts with FabHLH3 to regulate the late steps of anthocyanin and flavonol biosynthesis during ripening
Autor
Martínez Rivas, Félix Juan
Blanco Portales, Rosario
Pérez Serratosa, María
Ric-Varas, Pablo
Guerrero-Sánchez, Víctor M.
Medina Puche, Laura
Moyano Cañete, Lourdes
Mercado, José-Ángel
Alseekh, Saleh
Caballero, José Luis
Fernie, Alisdair R.
Muñoz-Blanco, Juan
Molina Hidalgo, Francisco Javier
Editor
WileyFecha
2023Materia
StrawberryFragaria
Fruit
Ripening
Transcription factor
Malonyltransferase
MYB
Anthocyanin/ flavonoid biosynthesis
Phenylpropanoid
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In this work, we identified and functionally characterized the strawberry (Fragaria × ananassa) R2R3 MYB transcription factor FaMYB123. As in most genes associated with organoleptic properties of ripe fruit, FaMYB123 expression is ripening-related, receptacle-specific, and antagonistically regulated by ABA and auxin. Knockdown of FaMYB123 expression by RNAi in ripe strawberry fruit receptacles downregulated the expression of enzymes involved in the late steps of anthocyanin/flavonoid biosynthesis. Transgenic fruits showed a parallel decrease in the contents of total anthocyanin and flavonoid, especially malonyl derivatives of pelargonidin and cyanidins. The decrease was concomitant with accumulation of proanthocyanin, propelargonidins, and other condensed tannins associated mainly with green receptacles. Potential coregulation between FaMYB123 and FaMYB10, which may act on different sets of genes for the enzymes involved in anthocyanin production, was explored. FaMYB123 and FabHLH3 were found to interact and to be involved in the transcriptional activation of FaMT1, a gene responsible for the malonylation of anthocyanin components during ripening. Taken together, these results demonstrate that FaMYB123 regulates the late steps of the flavonoid pathway in a specific manner. In this study, a new function for an R2R3 MYB transcription factor, regulating the expression of a gene that encodes a malonyltransferase, has been elucidated.