Tiempo de almacenamiento del medio cardio-condicionado y tipos de poliornitina, como factores limitantes para cultivos de ganglios ciliares disociados de embrión de pollo

Abstract

We have researched the effects of polyornithine (types IC and B) on neuron cultures dissociated from chick ciliary ganglia, incubated for 8 days in heart conditioned medium (HCM). Type IB is better as to axon elongation, and type IC for survival of neurons. The influence that time exerts on the inductive power of HCM was also studied employing polyornithine and collagen substrata. The results are not conclusive but there is some evidence that storing times longer than 18 days affect negativelg the HCM inductive potentiality on cultured neurons.

Hemos estudiado los efectos clue la poliornitina tipo IC y tipo IB ejercen sobre los cultivos de neuronas de ganglios ciliares disociados, de embriones he polio de echo dias de incubación, en presencia de medic) cardio-condicionado (MCC). El tipo IB favorece la elongation del axon y el tipo IC la supervivencia neuronal.