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A Straightforward Cytometry-Based Protocol for the Comprehensive Analysis of the Inflammatory Valve Infiltrate in Aortic Stenosis
Álvarez-Heredia, Pablo; Domínguez-del-Castillo, José Joaquín; Reina-Alfonso, Irene; Gutiérrez-González, Carmen; Hassouneh, Fakhri; Batista-Duharte, Alexander; Trujillo-Aguilera, Antonio; López-Romero, Rosalía; Muñoz, Ignacio; Pera, Alejandra (MDPI, 2023)Aortic stenosis (AS) is a frequent cardiac disease in old individuals, characterized by valvular calcification, fibrosis, and inflammation. Recent studies suggest that AS is an active inflammatory atherosclerotic-like ... -
Functional Changes of T-Cell Subsets with Age and CMV Infection
Hassouneh, Fakhri; Goldeck, David; Pera, Alejandra; Heemst, Diana van; Slagboom, P. Eline; Pawelec, Graham; Solana Lara, Rafael (MDPI, 2021)Cytomegalovirus (CMV) latent infection and aging contribute to alterations in the function and phenotype of the T-cell pool. We have demonstrated that CMV-seropositivity is associated with the expansion of polyfunctional ... -
Immune Checkpoint Inhibitors for Vaccine Improvements: Current Status and New Approaches
Batista-Duharte, Alexander; Hassouneh, Fakhri; Álvarez-Heredia, Pablo; Pera, Alejandra; Solana Lara, Rafael (MDPI, 2022)In recent years, the use of immune checkpoint inhibitors (ICIs) in combination with approved or experimental vaccines has proven to be a promising approach to improve vaccine immunogenicity and efficacy. This strategy seeks ...