Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin
Autor
Lozano Baena, María Dolores
Tasset, Inmaculada
Obregón Cano, Sara
Haro Bailón, Antonio de
Muñoz Serrano, Andrés
Alonso Moraga, Ángeles
Editor
MDPIFecha
2015Materia
Drosophila melanogasterHL60
Antigenotoxicity
Cytotoxicity
Brassica
Sinigrin
Bioactive compound
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Cruciferous vegetables are well known and worldwide consumed due to their
health benefits and cancer prevention properties. As a desirable cruciferous plant, Ethiopian
mustard (Brassica carinata A. Braun) and its glucosinolate sinigrin were tested in the in vivo
Drosophila melanogaster (SMART) and the in vitro HL60 (human promyelocytic leukaemia
cell line) systems. High performance liquid chromatography (HPLC) analysis of plant
samples confirmed the presence of sinigrin as principal B. carinata glucosinolate. SMART
was performed by feeding D. melanogaster larvae either with different concentrations of
plant/compound samples or combining them with hydrogen peroxide (a potent oxidative
mutagen) being both antimutagenics. HL60 assays showed the tumoricidal activity of
plant samples (IC50 = 0.28 mg·mL−1) and the breakdown products of sinigrin hydrolysis
(IC50 = 2.71 μM). Our results enhance the potential of B. carinata as health promoter and
chemopreventive in both systems and the leading role of sinigrin in these effects.