Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers
Autor
Tomás, Diana
Dias, Ana Luisa
Silva, Manuela
Oliveira, Hugo R.
Suso, María José
Viegas, Wanda
Veloso, María Manuela
Editor
MDPIFecha
2016Materia
Faba bean locally adapted populationsGenomic diversity
IRAP
METS:
Mostrar el registro METSPREMIS:
Mostrar el registro PREMISMetadatos
Mostrar el registro completo del ítemResumen
Faba bean have been grown in Portugal for a long time and locally adapted populations are
still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still
cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified
Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons
previously identified in other species can be efficiently used in the genetic variability assessment
of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns.
Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the
distribution pattern of V. faba samples. The four equina accessions are separated from each other and
form two distinct clades while the two major faba bean accessions are not unequivocally separated
by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP
Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome
coverage of this molecular marker. Morphological characteristics were also assessed through cluster
analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA)
and principal component analysis (PCA), showing a clear discrimination between faba bean major
and equina groups. It was also found that the seed character most relevant to distinguish accessions
was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting
the potential of IRAP analysis for genetic diversity studies.