Development of a Polyphenol Oxidase Biosensor from Jenipapo Fruit Extract (Genipa americana L.) and Determination of Phenolic Compounds in Textile Industrial Effluents
Autor
Souza Antunes, Rafael
Ferraz, Denes
Ferreira Garcia, Luane
Vieira Thomaz, Douglas
Luque, Rafael
Sanz Lobón, Germán
Souza Gil, Eric de
Marques Lopes, Flávio
Editor
MDPIFecha
2018Materia
Genipa americana L.Polifenoloxidases
Biosensor enzyme
Phenolic compounds
METS:
Mostrar el registro METSPREMIS:
Mostrar el registro PREMISMetadatos
Mostrar el registro completo del ítemResumen
In this work, an innovative polyphenol oxidase biosensor was developed from Jenipapo
(Genipa americana L.) fruit and used to assess phenolic compounds in industrial effluent samples
obtained from a textile industry located in Jaraguá-GO, Brasil. The biosensor was prepared and
optimized according to: the proportion of crude vegetal extract, pH and overall voltammetric
parameters for differential pulse voltammetry. The calibration curve presented a linear interval from
10 to 310 M (r2 = 0.9982) and a limit of detection of 7 M. Biosensor stability was evaluated
throughout 15 days, and it exhibited 88.22% of the initial response. The amount of catechol
standard recovered post analysis varied between 87.50% and 96.00%. Moreover, the biosensor
was able to detect phenolic compounds in a real sample, and the results were in accordance with
standard spectrophotometric assays. Therefore, the innovatively-designed biosensor hereby proposed
is a promising tool for phenolic compound detection and quantification when environmental
contaminants are concerned