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dc.contributor.authorCebrián-García, Soledad
dc.contributor.authorBalu, Alina M.
dc.contributor.authorGarcía, Araceli
dc.contributor.authorLuque, Rafael
dc.date.accessioned2018-11-02T09:43:18Z
dc.date.available2018-11-02T09:43:18Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/10396/17396
dc.description.abstractAlkyl esters are high added value products useful in a wide range of industrial sectors. A methodology based on a simple sol-gel approach (biosilicification) is herein proposed to encapsulate enzymes in order to design highly active and stable biocatalysts. Their performance was assessed through the optimization of valeric acid esterification evaluating the effect of different parameters (biocatalyst load, presence of water, reaction temperature and stirring rate) in different alcoholic media, and comparing two different methodologies: conventional heating and microwave irradiation. Ethyl valerate yields were in the 80–85% range under optimum conditions (15 min, 12% m/v biocatalyst, molar ratio 1:2 of valeric acid to alcohol). Comparatively, the biocatalysts were slightly deactivated under microwave irradiation due to enzyme denaturalisation. Biocatalyst reuse was attempted to prove that good reusability of these sol-gel immobilised enzymes could be achieved under conventional heating.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceMolecules 23, 2283 (2018)es_ES
dc.subjectSol-gel biosilicificationes_ES
dc.subjectEsterificationes_ES
dc.subjectConventional heatinges_ES
dc.subjectMicrowaveses_ES
dc.titleSol-Gel Immobilisation of Lipases: Towards Active and Stable Biocatalysts for the Esterification of Valeric Acides_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/molecules23092283es_ES
dc.relation.projectIDGobierno de España. CTQ2016-78289-Pes_ES
dc.relation.projectIDGobierno de España. IJCI-2015-23168es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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