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dc.contributor.authorRíos, Rafael
dc.contributor.authorRaya, Ana I.
dc.contributor.authorPineda Martos, Carmen María
dc.contributor.authorRodríguez, Mariano
dc.contributor.authorLópez, Ignacio
dc.contributor.authorAguilera Tejero, Escolástico
dc.date.accessioned2018-12-03T13:32:44Z
dc.date.available2018-12-03T13:32:44Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10396/17552
dc.description.abstractBackground: High fat diets are implicated in the pathogenesis of metabolic syndrome, obesity and renal disease. Previous studies have revealed that high fat diets promote vascular calcification in uremic rats. Moreover, vitamin E has been shown to prevent uremic calcifications in genetically obese Zucker rats fed standard diet. The objective of this study was to investigate the influence of vitamin E supplementation on the development of extraskeletal calcifications in non-obese (wild type) uremic rats fed high fat diets. Methods: Wistar rats ( n = 32) were preconditioned by feeding either a normal (NF) or high fat (HF) diet for 45 days and subsequently were subjected to 5/6 nephrectomy (Nx). Just before performing the first Nx step, a blood sample (Pre-Nx) was obtained. After Nx rat s were switched to a diet with 0.9% phosphorus and supplemented with calcitriol. Also, after Nx, half of the rats from each group (NF and HF) were treated with vitamin E (VitE) in the diet (30,000 mg/kg) and the ot her half were maintained on basic VitE requirements (27 mg/kg). Thus, rats were allotted to four experimental groups: Nx-NF ( n = 8), Nx-NF-VitE ( n =8),Nx-HF( n =8)and Nx-HF-VitE ( n = 8). At the time of sacrifice (day 66), blood and tissue samples were obtained. Results: Feeding a HF diet for 45 days did not increase body weight but elicited hyperglycemia, hypertriglyceridemia, an increase in plasma fibroblast growth factor 23 and a reduction in plasma calcitriol concentrations. After Nx, rats fed HF diet showed substantial extraskeletal calcification with aortic calcium content that was higher than in rats fed NF diet. Supplementation with VitE significantly ( p < 0.05) reduced aortic (from 38.4 ± 8.8 to 16.5 ± 1.4 mg/g), gastric (from 5.6 ± 2.7 to 1.2 ± 0.4 mg/g) and pulmonary (from 1.8 ± 0.3 to 0.3 ± 0.2 mg/g) calcium content in rats on HF diets. Conclusions: Uremic rats fed HF diets developed more severe extraosseous calcifications than their normocaloric-fed counterparts and dietary VitE supplementation protected against uremic calcifications in rats fed HF diets. Thus, eating energy-rich foods should be discouraged in patients with renal disease and their deleterious effect may be ameliorated with adequate antioxidant supplyes_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceBMC Nephrology 18:374 (2017)es_ES
dc.subjectVascular calcificationes_ES
dc.subjectUremiaes_ES
dc.subjectHigh-fat dietes_ES
dc.subjectVitamin Ees_ES
dc.titleVitamin E protects against extraskeletal calcification in uremic rats fed high fat dietses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.1186/s12882-017-0790-4es_ES
dc.relation.projectIDGobierno de España. PI14/00467es_ES
dc.relation.projectIDGobierno de España. PI14/00638es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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