Listar DGen-Artículos, capítulos... por autor "cb4e0018-c80f-44b8-9fc0-0654e2d683c9"
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Advent of Genomics in Blueberry
Die, Jose V.; Rowland, Lisa J. (Springer, 2013)Blueberry is a high value crop with recognized nutritional characteristics that has lead to an increase in consumer demand over the last several years. With its increasing agricultural and commercial importance, genetic ... -
Aldehyde Dehydrogenase 3 Is an Expanded Gene Family with Potential Adaptive Roles in Chickpea
Carmona Molero, Rocío; Jiménez-López, José C.; Caballo Linares, Cristina; Gil, Juan; Millán, Teresa; Die, Jose V. (MDPI, 2021)Legumes play an important role in ensuring food security, improving nutrition and enhancing ecosystem resilience. Chickpea is a globally important grain legume adapted to semi-arid regions under rain-fed conditions. A ... -
Characterization and Analysis of Anthocyanin-Related Genes in Wild-Type Blueberry and the Pink-Fruited Mutant Cultivar ‘Pink Lemonade’: New Insights into Anthocyanin Biosynthesis
Die, Jose V.; Jones, Richard W.; Ogden, Elizabeth L.; Ehlenfeldt, Mark K.; Rowland, Lisa J. (MDPI, 2020)Blueberries are one of the richest sources of antioxidants, such as anthocyanins, among fruits and vegetables. Anthocyanin mutants, like the pink-fruited cultivar ‘Pink Lemonade’, are valuable resources for investigating ... -
Characterization of the 3’: 5’ ratio for reliable determination of RNA quality
Die, Jose V.; Obrero, Ángeles; González-Verdejo, Clara I.; Román, Belén (Elsevier, 2011)Determination of RNA quality is a critical first step in obtaining meaningful gene expression data. The PCR-based 3’: 5’ assay is an RNA quality assessment tool. This assay is a simple, fast and low-cost method of selecting ... -
Design and sampling plan optimization for RT-qPCR experiments in plants: a case study in blueberry
Die, Jose V.; Román, Belén; Flores, Fernando; Rowland, Lisa J. (Frontiers, 2016)The qPCR assay has become a routine technology in plant biotechnology and agricultural research. It is unlikely to be technically improved, but there are still challenges which center around minimizing the variability ... -
Efficient single nucleotide polymorphism marker-assisted selection to fusarium wilt in chickpea
Castro, Patricia; Caballo, Cristina; Carmona, A.; Millán, Teresa; Gil, Juan; Die, Jose V.; Izquierdo, Inmaculada; Rubio, Josefa (MDPI, 2024)Abstract Fusarium wilt is one of the most destructive chickpea diseases worldwide. Race 5 (Foc5) is the most harmful in the Mediterranean basin. The primary objective of this study is to validate a block of six SNP markers ... -
Elucidating cold acclimation pathway in blueberry by transcriptome profiling
Die, Jose V.; Rowland, Lisa J. (Elsevier, 2014)A fundamental goal of cold acclimation research is to understand the mechanisms responsible for the increase in freezing tolerance in response to environmental cues. Changes in gene expression underlie some of the biochemical ... -
Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions
Die, Jose V.; Román, Belén; Nadal, Salvador; González-Verdejo, Clara I. (Springer, 2010)Real-time reverse transcriptase quantitative polymerase chain reaction is the most accurate measure of gene expression in biological systems. The data is analyzed through a process called normalization. Internal standards ... -
Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection
Die, Jose V.; Román, Belén; Nadal, Salvador; Dita, Miguel; González-Verdejo, Clara I. (CSIRO, 2009)The root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental ... -
Gene expression analysis of molecular mechanisms of defense induced in Medicago truncatula parasitized by Orobanche crenata
Die, Jose V.; González-Verdejo, Clara I.; Dita, Miguel; Nadal, Salvador; Román, Belén (Elsevier, 2009)The infection of Medicago truncatula Gaertn. roots with the obligate parasite Orobanche crenata Forsk. is a useful model for studying the molecular events involved in the legumes–parasite interaction. In order to gain ... -
Gene expression profiling of Medicago truncatula roots in response to the parasitic plant Orobanche crenata
Dita, Miguel; Die, Jose V.; Román, Belén; Krajinski, Franziska; Küster, H.; Moreno, Mª Teresa; Cubero, José Ignacio (Wiley, 2009)Orobanche crenata is a root parasitic weed that is a major constraint for grain and forage legume cultivation in Mediterranean and West Asia. Only moderate to low levels of incomplete resistance of complex inheritance has ... -
Global patterns of protein abundance during the development of cold hardiness in blueberry
Die, Jose V.; Rajeev, Arora; Rowland, Lisa J. (Elsevier, 2016)To gain a better understanding of the cold acclimation progression in blueberry, we investigated the proteome-level changes that occur in flower buds with increasing exposure to chilling temperatures using the 2D-DIGE ... -
Identification by suppression subtractive hybridization and expression analysis of Medicago truncatula putative defence genes in response to Orobanche crenata parasitization
Die, Jose V.; Dita, Miguel; Krajinski, Franziska; González-Verdejo, Clara I.; Rubiales, Diego; Moreno, Mª Teresa; Román, Belén (Elsevier, 2007)Crenate broomrape (Orobanche crenata) is the major constraint for pea and faba bean production in the Mediterranean region. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a ... -
QTL Analysis of Morpho-Agronomic Traits in Garden Asparagus (Asparagus officinalis L.)
García, Verónica; Castro, Patricia; Die, Jose V.; Millán, Teresa; Gil, Juan; Moreno, Roberto (MDPI, 2023)In order to understand the genetic control of quantitative agronomic traits in garden asparagus, we performed a quantitative trait loci (QTL) analysis. A population (n = 167) derived from a cross between a female and male ... -
RNA quality assessment: a view from plant qPCR studies
Die, Jose V.; Román, Belén (Oxford University Press, 2012)Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is probably the most common molecular technique used in transcriptome analyses today. The simplicity of the technology and associated protocols ... -
Segmental and Tandem Duplications Driving the Recent NBS-LRR Gene Expansion in the Asparagus Genome
Die, Jose V.; Castro, Patricia; Millán, Teresa; Gil, Juan (MDPI, 2018)Garden asparagus is an important horticultural plant worldwide. It is, however, susceptible to a variety of diseases, which can affect the potential yield, spear quality, and lifespan of production fields. Screening studies ... -
Selection of housekeeping genes for normalization by real-time RT–PCR: Analysis of Or-MYB1 gene expression in Orobanche ramosa development
González-Verdejo, Clara I.; Die, Jose V.; Nadal, Salvador; Jiménez-Marín, Ángeles; Moreno, Mª Teresa; Román, Belén (Elsevier, 2008)Real-time PCR has become the method of choice for accurate and in-depth expression studies of candidate genes. To avoid bias, real-time PCR is referred to one or several internal control genes that should not fluctuate ... -
Superior Cross-Species Reference Genes: A Blueberry Case Study
Die, Jose V.; Rowland, Lisa J. (Public Library of Science (PLOS), 2013)The advent of affordable Next Generation Sequencing technologies has had major impact on studies of many crop species, where access to genomic technologies and genome-scale data sets has been extremely limited until now. ...