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dc.contributor.authorDorado, G.
dc.contributor.authorGálvez, Sergio
dc.contributor.authorRosales, Teresa E.
dc.contributor.authorVásquez, Víctor F.
dc.contributor.authorHernández, Pilar
dc.date.accessioned2021-07-28T10:56:59Z
dc.date.available2021-07-28T10:56:59Z
dc.date.issued2021
dc.identifier.urihttp://hdl.handle.net/10396/21544
dc.description.abstractRecent developments have revolutionized the study of biomolecules. Among them are molecular markers, amplification and sequencing of nucleic acids. The latter is classified into three generations. The first allows to sequence small DNA fragments. The second one increases throughput, reducing turnaround and pricing, and is therefore more convenient to sequence full genomes and transcriptomes. The third generation is currently pushing technology to its limits, being able to sequence single molecules, without previous amplification, which was previously impossible. Besides, this represents a new revolution, allowing researchers to directly sequence RNA without previous retrotranscription. These technologies are having a significant impact on different areas, such as medicine, agronomy, ecology and biotechnology. Additionally, the study of biomolecules is revealing interesting evolutionary information. That includes deciphering what makes us human, including phenomena like non-coding RNA expansion. All this is redefining the concept of gene and transcript. Basic analyses and applications are now facilitated with new genome editing tools, such as CRISPR. All these developments, in general, and nucleic-acid sequencing, in particular, are opening a new exciting era of biomolecule analyses and applications, including personalized medicine, and diagnosis and prevention of diseases for humans and other animals.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceBiomolecules 11(8), 1111 (2021)es_ES
dc.subjectFirst-generation sequencing (FGS)es_ES
dc.subjectSecond-generation sequencing (SGS)es_ES
dc.subjectThird-generation sequencing (TGS)es_ES
dc.subjectHigh-throughput sequencing (HTS)es_ES
dc.subjectNext-generation sequencing (NGS)es_ES
dc.subjectStructural genomicses_ES
dc.subjectFunctional genomicses_ES
dc.subjectEpigenomicses_ES
dc.subjectMetagenomicses_ES
dc.titleAnalyzing Modern Biomolecules: The Revolution of Nucleic-Acid Sequencing-Reviewes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/biom11081111es_ES
dc.relation.projectIDGobierno de España. BIO2015-64737-Res_ES
dc.relation.projectIDGobierno de España. RF 2012-00002-C2-02es_ES
dc.relation.projectIDJunta de Andalucía. 041/C/2007es_ES
dc.relation.projectIDJunta de Andalucía. 75/C/2009es_ES
dc.relation.projectIDJunta de Andalucía. 56/C/2010es_ES
dc.relation.projectIDJunta de Andalucía. P11-AGR-7322es_ES
dc.relation.projectIDJunta de Andalucía. P18-RT-992es_ES
dc.relation.projectIDJunta de Andalucía. AGR-248es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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