Listar DBBM-Artículos, capítulos... por autor "0d68c1f2-a493-4f7f-ad6f-4936bf3a1454"
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Alternative pathway for 3-cyanoalanine assimilation in pseudomonas pseudoalcaligenes CECT5344 under Noncyanotrophic conditions
Pérez, María Dolores; Olaya-Abril, Alfonso; Cabello, Purificación; Sáez, Lara P.; Roldán, María Dolores; Moreno-Vivián, Conrado; Luque-Almagro, Víctor Manuel (American Society for Microbiology, 2021)3-Cyanoalanine and cyanohydrins are intermediate nitriles produced in cyanide degradation pathways in plants and bacteria. 3-Cyanoalanine is generated from cyanide by the 3-cyanoalanine synthase, an enzyme mainly characterized ... -
Approaching In Vivo Models of Pneumococcus–Host Interaction: Insights into Surface Proteins, Capsule Production, and Extracellular Vesicles
Olaya-Abril, Alfonso; González Reyes, José Antonio; Rodríguez-Ortega, Manuel J. (MDPI, 2021)Infections caused by the Gram-positive bacterium Streptococcus pneumoniae have become a major health problem worldwide because of their high morbidity and mortality rates, especially in developing countries. This microorganism ... -
Assimilation of cyanide and cyano-derivatives by Pseudomonas pseudoalcaligenes CECT5344: from omic approaches to biotechnological applications
Cabello, Purificación; Luque-Almagro, Víctor Manuel; Olaya-Abril, Alfonso; Sáez, Lara P.; Moreno-Vivián, Conrado; Roldán, María Dolores (Oxford Academic, 2018)Mining, jewellery and metal-processing industries use cyanide for extracting gold and other valuable metals, generating large amounts of highly toxic wastewater. Biological treatments may be a clean alternative under the ... -
Bioinformática como recurso educativo: Proyecto de ingeniería genética
Olaya-Abril, Alfonso; Cejas Molina, M.A. (Universidad de Córdoba, UCOPress, 2018)La utilización de medios informáticos para procesar los contenidos curriculares de las diferentes materias, así como para aprender su propio manejo, ha crecido exponencialmente en los últimos años. El uso de las Tecnologías ... -
Bioremediation of cyanide-containing wastes: The potential of systems and synthetic biology for cleaning up the toxic leftovers from mining
Roldán Alfonso, María Dolores; Olaya-Abril, Alfonso; Sáez, Lara P.; Cabello, Purificación; Luque-Almagro, Víctor Manuel; Moreno-Vivián, Conrado (Wiley, 2021)Cyanide is a highly toxic chemical produced in large amounts by the mining and jewellery industries, steel manufacturing, coal coking, food processing and chemical synthesis (Luque-Almagro et al, 2011). The mining industry ... -
Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
Olaya-Abril, Alfonso; Hidalgo-Carrillo, J.; Luque-Almagro, Víctor Manuel; Fuentes-Almagro, Carlos; Urbano, Francisco José; Moreno-Vivián, Conrado; Richardson, David J.; Roldán Alfonso, María Dolores (Nature, 2021)Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which ... -
Exploring anaerobic environments for cyanide and cyano-derivatives microbial degradation
Luque-Almagro, Víctor Manuel; Cabello, Purificación; Sáez, Lara P.; Olaya-Abril, Alfonso; Moreno-Vivián, Conrado; Roldán, María Dolores (SpringerLink, 2018)Cyanide is one of the most toxic chemicals for living organisms described so far. Its toxicity is mainly based on the high affinity that cyanide presents toward metals, provoking inhibition of essential metalloenzymes. ... -
Exploring the denitrification proteome of paracoccus denitrificans PD1222
Olaya-Abril, Alfonso; Hidalgo-Carrillo, J.; Luque-Almagro, Víctor Manuel; Fuentes-Almagro, Carlos; Urbano, Francisco José; Moreno-Vivián, Conrado; Richardson, David J.; Roldán, María Dolores (Frontiers, 2018)Denitrification is a respiratory process that produces nitrous oxide as an intermediate, which may escape to the atmosphere before its reduction to dinitrogen through the nitrous oxide reductase NosZ. In this work, the ... -
Extracellular Vesicles from Different Pneumococcal Serotypes Are Internalized by Macrophages and Induce Host Immune Responses
Olaya-Abril, Alfonso; Prados Rosales, Rafael; González Reyes, José Antonio; Casadevall, Arturo; Pirofski, Liise-anne; Rodríguez-Ortega, Manuel J. (MDPI, 2021)Bacterial extracellular vesicles are membranous ultrastructures released from the cell surface. They play important roles in the interaction between the host and the bacteria. In this work, we show how extracellular vesicles ... -
Genomic Insights into Cyanide Biodegradation in the Pseudomonas Genus
Sáez, Lara P.; Rodríguez Caballero, Gema; Olaya-Abril, Alfonso; Cabello, Purificación; Moreno-Vivián, Conrado; Roldán, María Dolores; Luque-Almagro, Víctor Manuel (MDPI, 2024)Molecular studies about cyanide biodegradation have been mainly focused on the hydrolytic pathways catalyzed by the cyanide dihydratase CynD or the nitrilase NitC. In some Pseudomonas strains, the assimilation of cyanide ... -
Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003
Biełło, Karolina A.; Lucena, Carlos; López Tenllado, Francisco Javier; Hidalgo-Carrillo, J.; Rodríguez Caballero, Gema; Cabello, Purificación; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Moreno-Vivián, Conrado; Olaya-Abril, Alfonso (Frontiers, 2023)Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield and quality. N and P chemical fertilizers are widely used in current agriculture, causing ... -
Identification of new immunoprotective surface protein vaccine candidates against streptococcus suis infection by proteomics
Gómez-Gascón, Lidia; Olaya-Abril, Alfonso; Jiménez-Munguía, I.; Luque-Moreno, Inmaculada; Tarradas-Iglesias, Carmen; Bárcena Ruiz, José Antonio; Rodríguez-Ortega, Manuel J. (Universidad de Córdoba, Servicio de Publicaciones, 2011) -
Identification of Potential New Protein Vaccine Candidates through Pan-Surfomic Analysis of Pneumococcal Clinical Isolates from Adults
Olaya-Abril, Alfonso; Jiménez-Munguía, I.; Gómez-Gascón, Lidia; Obando, Ignacio; Rodríguez-Ortega, Manuel J. (Public Library of Science, 2013)Purified polysaccharide and conjugate vaccines are widely used for preventing infections in adults and in children against the Gram-positive bacterium Streptococcus pneumoniae, a pathogen responsible for high morbidity ... -
Poly(3-hydroxybutyrate) hyperproduction by a global nitrogen regulator NtrB mutant strain of Paracoccus denitrificans PD1222
Olaya-Abril, Alfonso; Luque-Almagro, Víctor Manuel; Manso, Isabel; Gates, Andrew J.; Moreno-Vivián, Conrado; Richardson, David J.; Roldán, María Dolores (Oxford Academic, 2017)Paracoccus denitrificans PD1222 accumulates short-length polyhydroxyalkanoates, poly(3-hydroxybutyrate), under nitrogen-deficient conditions. Polyhydroxybutyrate metabolism requires the 3-ketoacyl-CoA thiolase PhaA, the ... -
Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344
Biełło, Karolina A.; Cabello, Purificación; Rodríguez Caballero, Gema; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Olaya-Abril, Alfonso; Moreno-Vivián, Conrado (MDPI, 2023)Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the ... -
Quantitative proteomic analysis of cyanide and mercury detoxification by pseudomonas pseudoalcaligenes CECT 5344
Biełło, Karolina A.; Olaya-Abril, Alfonso; Cabello, Purificación; Rodríguez Caballero, Gema; Sáez, Lara P.; Moreno-Vivián, Conrado; Luque-Almagro, Víctor Manuel; Roldán, María Dolores (American Society for Microbiology, 2023)The cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT 5344 uses cyanide and different metal-cyanide complexes as the sole nitrogen source. Under cyanotrophic conditions, this strain was able to grow with up ... -
Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-contain ing wastewaters using Liquid Chromatography- Mass Spectrometry/Mass Spectrometry (LC- MS/MS)
Ibáñez, María Isabel; Cabello, Purificación; Luque-Almagro, Víctor Manuel; Sáez, Lara P.; Olaya-Abril, Alfonso; Sánchez de Medina, Verónica; Luque de Castro, M.D.; Moreno-Vivián, Conrado; Roldán, María Dolores (PLOS, 2017)Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas ps ... -
The NtrYX Two-Component System of Paracoccus denitrificans Is Required for the Maintenance of Cellular Iron Homeostasis and for a Complete Denitrification under Iron-Limited Conditions
Olaya-Abril, Alfonso; Luque-Almagro, Víctor Manuel; Hidalgo-Carrillo, J.; Chicano-Gálvez, Eduardo; Urbano, Francisco José; Moreno-Vivián, Conrado; Richardson, David J.; Roldán, María Dolores (MDPI, 2022)Denitrification consists of the sequential reduction of nitrate to nitrite, nitric oxide, nitrous oxide, and dinitrogen. Nitrous oxide escapes to the atmosphere, depending on copper availability and other environmental ...