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Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy
(MDPI, 2020)
Unlike orthodox species, seed recalcitrance is poorly understood, especially at the molecular level. In this regard, seed maturation and germination were studied in the non-orthodox Quercus ilex by using a proteomics ...
Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?
(MDPI, 2019)
Proteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as Quercus, Pines, Poplars, and Eucalyptus. This review assesses the potential and limitations of the proteomics ...
Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green Alga Chlamydomonasreinhardtii
(MDPI, 2020)
The mitogen activated protein kinases (MAPKs) form part of a signaling cascade through phosphorylation reactions conserved in all eukaryotic organisms. The MAPK cascades are mainly composed by threeproteins, MAPKKKs, MAPKKs ...
Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae
(MDPI, 2020)
Low stability of transgenes and high variability of their expression levels among the obtained transformants are still pending challenges in the nuclear genetic transformation of microalgae. We have generated a new ...
Chlamydomonas reinhardtii, an Algal Model in the Nitrogen Cycle
(MDPI, 2020)
Nitrogen (N) is an essential constituent of all living organisms and the main limiting macronutrient. Even when dinitrogen gas is the most abundant form of N, it can only be used by fixing bacteria but is inaccessible to ...
Responses and Differences in Tolerance to Water Shortage under Climatic Dryness Conditions in Seedlings from Quercus spp. and Andalusian Q. ilex Populations
(MDPI, 2020)
Analyzing differences in tolerance to drought in Quercus spp., and the characterization of these responses at the species and individual population level, are imperative for the selection of resilient elite genotypes in ...
Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster
(MDPI, 2019)
The alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 can grow with cyanate, cyanide, or cyanide-containing industrial residues as the sole nitrogen source, but the assimilation of cyanide and cyanate takes ...
Population Genetic Diversity of Quercus ilex subsp. ballota (Desf.) Samp. Reveals Divergence in Recent and Evolutionary Migration Rates in the Spanish Dehesas
(MDPI, 2018)
The Spanish dehesas have been severely affected by human activities that date to the
prehistoric period and have suffered accelerated decline since the 1980s. Holm oak (Quercus ilex
subsp. ballota (Desf.) Samp.) is a key ...
Characterization of the Burkholderia cenocepacia J2315 Surface-Exposed Immunoproteome
(MDPI, 2020)
Infections by the Burkholderia cepacia complex (Bcc) remain seriously life threatening to cystic fibrosis (CF) patients, and no effective eradication is available. A vaccine to protect patients against Bcc infections is a ...
Study of Different Variants of Mo Enzyme crARC and the Interaction with Its Partners crCytb5-R and crCytb5-1
(MDPI, 2017)
The mARC (mitochondrial Amidoxime Reducing Component) proteins are recently
discovered molybdenum (Mo) Cofactor containing enzymes. They are involved in the reduction
of several N-hydroxylated compounds (NHC) and nitrite. ...