ListarArtículos, capítulos, libros...UCO por tema "qPCR"
Mostrando ítems 1-7 de 7
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Aldehyde Dehydrogenase 3 Is an Expanded Gene Family with Potential Adaptive Roles in Chickpea
(MDPI, 2021)Legumes play an important role in ensuring food security, improving nutrition and enhancing ecosystem resilience. Chickpea is a globally important grain legume adapted to semi-arid regions under rain-fed conditions. A ... -
Characterization and Analysis of Anthocyanin-Related Genes in Wild-Type Blueberry and the Pink-Fruited Mutant Cultivar ‘Pink Lemonade’: New Insights into Anthocyanin Biosynthesis
(MDPI, 2020)Blueberries are one of the richest sources of antioxidants, such as anthocyanins, among fruits and vegetables. Anthocyanin mutants, like the pink-fruited cultivar ‘Pink Lemonade’, are valuable resources for investigating ... -
Characterization of the 3’: 5’ ratio for reliable determination of RNA quality
(Elsevier, 2011)Determination of RNA quality is a critical first step in obtaining meaningful gene expression data. The PCR-based 3’: 5’ assay is an RNA quality assessment tool. This assay is a simple, fast and low-cost method of selecting ... -
Design and sampling plan optimization for RT-qPCR experiments in plants: a case study in blueberry
(Frontiers, 2016)The qPCR assay has become a routine technology in plant biotechnology and agricultural research. It is unlikely to be technically improved, but there are still challenges which center around minimizing the variability ... -
Elucidating cold acclimation pathway in blueberry by transcriptome profiling
(Elsevier, 2014)A fundamental goal of cold acclimation research is to understand the mechanisms responsible for the increase in freezing tolerance in response to environmental cues. Changes in gene expression underlie some of the biochemical ... -
RNA quality assessment: a view from plant qPCR studies
(Oxford University Press, 2012)Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is probably the most common molecular technique used in transcriptome analyses today. The simplicity of the technology and associated protocols ... -
Superior Cross-Species Reference Genes: A Blueberry Case Study
(Public Library of Science (PLOS), 2013)The advent of affordable Next Generation Sequencing technologies has had major impact on studies of many crop species, where access to genomic technologies and genome-scale data sets has been extremely limited until now. ...