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dc.contributor.authorGarcía, Mauro D.
dc.contributor.authorRuiz, María J.
dc.contributor.authorMedina, Luis M.
dc.contributor.authorVidal, Roberto
dc.contributor.authorPadola, Nora L.
dc.contributor.authorEtcheverria, Analía I.
dc.date.accessioned2023-07-14T09:03:10Z
dc.date.available2023-07-14T09:03:10Z
dc.date.issued2023
dc.identifier.issnISSN: 2304-8158
dc.identifier.urihttp://hdl.handle.net/10396/25761
dc.description.abstractThe objective of this work was to molecularly and genotypically characterize and test the inhibitory activity of six colicinogenic Escherichia coli strains (ColEc) and their partially purified colicins against STEC O157:H7 isolated from clinical human cases. Inhibition tests demonstrated the activity of these strains and their colicins against STEC O157:H7. By PCR it was possible to detect colicins Ia, E7, and B and microcins M, H47, C7, and J25. By genome sequencing of two selected ColEc strains, it was possible to identify additional colicins such as E1 and Ib. No genes coding for stx1 and stx2 were detected after analyzing the genome sequence. The inhibitory activity of ColEc against STEC O157:H7 used as an indicator showed that colicins are potent growth inhibitors of E. coli O157:H7, being a potential alternative to reduce the presence of pathogens of public health relevance.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceFoods, 12(14), 2676 (2023)es_ES
dc.subjectSTECes_ES
dc.subjectE. coli O157:H7es_ES
dc.subjectColicines_ES
dc.subjectGenomees_ES
dc.titleMolecular and genetic characterization of colicinogenic Escherichia coli strains active against Shiga toxin-producing Escherichia coli O157:H7es_ES
dc.typeinfo:eu-repo/semantics/article
dc.relation.publisherversionhttps://doi.org/10.3390/foods12142676es_ES
dc.relation.projectIDGobierno de España. PICT2015-2666es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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