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dc.contributor.advisorPrieto Aranda, Pilar
dc.contributor.authorRey Santome, María Dolores
dc.date.accessioned2015-03-19T08:40:50Z
dc.date.available2015-03-19T08:40:50Z
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10396/12657
dc.description.abstractInterspecific hybridization is used to introduce desirable characters from related species into allopolyploids such as wheat. The development of substitution and addition lines of related species in bread wheat can be useful as a genetic tool to transfer agronomic traits in the background of bread wheat. However, there is a low level of pairing and recombination between wheat chromosomes and those from the relative species. Different approaches have been carried out to promote pairing and recombination between related chromosomes such as the ph1b mutant, ionizing radiations and gametocidal genes. However, the most important discovery has been the Ph1 locus which regulates pairing and recombination between homologous chromosomes during meiosis in wheat. Thus, in its absence, related chromosomes could associate and recombine. Chapter 2 of this work examines the dynamic of DNA replication during premeiosis and early meiosis in wheat using flow cytometry, which has allowed the quantification of the amount of DNA in wheat anther in each stage meiotic stage. Chromosome replication was detected in wheat during premeiosis and early meiosis until the stage of pachytene, when chromosomes are associated in pairs to further recombine and correctly segregate in the gametes. Also, an important role of the Ph1 locus on the length of meiotic DNA replication in wheat was shown using flow cytometry. Chapter 3 deals with chromosome manipulation to induce meiotic recombination between barley and wheat in the absence of the Ph1 locus. Genetic crosses between the ph1b mutant and both wild and cultivated barley substitution and addition lines in wheat were carried out. More than...es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherUniversidad de Córdoba, UCOPresses_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.subjectWheates_ES
dc.subjectBarleyes_ES
dc.subjectDNAes_ES
dc.subjectPremeiosises_ES
dc.subjectMeiosises_ES
dc.subjectChromosome manipulationes_ES
dc.subjectGenetic introgessionses_ES
dc.titleStudy and characterization of the Ph1 locus as tool to promote interspecific chromosome associations between wheat and barley specieses_ES
dc.typeinfo:eu-repo/semantics/doctoralThesises_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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