Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary Tuberculosis

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Author
Causse, Manuel
Ruiz, Pilar
Gutiérrez-Aroca, Juan-Bautista
Casal Román, Manuel
Publisher
American Society for MicrobilogyDate
2011Subject
M. tuberculosisReal-time PCR
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Show full item recordAbstract
Application of real-time PCR for the detection of
Mycobacterium tuberculosis
enables results to be obtained
in about 2 h. A total of 340 nonrespiratory samples were processed using two real-time PCR assay kits: Xpert
MTB/RIF and Cobas TaqMan MTB. The sensitivity and specificity of the Xpert assay were 95% and 100%,
respectively, compared to 78% and 98% for the Cobas assay.