Listar Departamento de Bioquímica y Biología Molecular por autor "33261a55-74b5-4a98-8641-36e4dcfe2651"
Mostrando ítems 1-19 de 19
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Alternative pathway for 3-cyanoalanine assimilation in pseudomonas pseudoalcaligenes CECT5344 under Noncyanotrophic conditions
Pérez, María Dolores; Olaya-Abril, Alfonso; Cabello, Purificación; Sáez, Lara P.; Roldán, María Dolores; Moreno-Vivián, Conrado; Luque-Almagro, Víctor Manuel (American Society for Microbiology, 2021)3-Cyanoalanine and cyanohydrins are intermediate nitriles produced in cyanide degradation pathways in plants and bacteria. 3-Cyanoalanine is generated from cyanide by the 3-cyanoalanine synthase, an enzyme mainly characterized ... -
Assimilation of cyanide and cyano-derivatives by Pseudomonas pseudoalcaligenes CECT5344: from omic approaches to biotechnological applications
Cabello, Purificación; Luque-Almagro, Víctor Manuel; Olaya-Abril, Alfonso; Sáez, Lara P.; Moreno-Vivián, Conrado; Roldán, María Dolores (Oxford Academic, 2018)Mining, jewellery and metal-processing industries use cyanide for extracting gold and other valuable metals, generating large amounts of highly toxic wastewater. Biological treatments may be a clean alternative under the ... -
Biodegradación de cianuro por Pseudomonas pseudoalcaligenes CECT5344. Análisis proteómico
Huertas, M. José; Luque-Almagro, Víctor Manuel; Martínez-Luque, M.; García, I.; Blasco, R.; Castillo, F.; Roldán, María Dolores; Moreno-Vivián, Conrado (Universidad de Córdoba, 2009)El cianuro es un compuesto muy tóxico para los seres vivos ya que forma complejos muy estables con metales de transición, esenciales para la función de numerosas proteínas. A pesar de su toxicidad, se han descrito ... -
Bioremediation of cyanide-containing wastes: The potential of systems and synthetic biology for cleaning up the toxic leftovers from mining
Roldán Alfonso, María Dolores; Olaya-Abril, Alfonso; Sáez, Lara P.; Cabello, Purificación; Luque-Almagro, Víctor Manuel; Moreno-Vivián, Conrado (Wiley, 2021)Cyanide is a highly toxic chemical produced in large amounts by the mining and jewellery industries, steel manufacturing, coal coking, food processing and chemical synthesis (Luque-Almagro et al, 2011). The mining industry ... -
Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster
Sáez, Lara P.; Cabello, Purificación; Ibáñez, María Isabel; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Moreno-Vivián, Conrado (MDPI, 2019)The alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 can grow with cyanate, cyanide, or cyanide-containing industrial residues as the sole nitrogen source, but the assimilation of cyanide and cyanate takes ... -
Cyanide biodegradation by a native bacterial consortium and its potential for goldmine tailing biotreatment
Alvarado-López, María José; Garrido-Hoyos, Sofía E.; Raynal-Gutiérrez, María Elena; El-Kassis, Elie G.; Luque-Almagro, Víctor Manuel; Rosano-Ortega, Genoveva (MDPI, 2023)A native cyanide-degrading bacterial consortium was isolated from goldmine tailing sediments. Mine tailings are toxic effluents due to their metal–cyanide complexes. The bacterial consortium was able to degrade an initial ... -
DNA microarray analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to nitrogen starvation, cyanide and a jewelry wastewater
Luque-Almagro, Víctor Manuel; Escribano Fernández, María de la Paz; Manso Cobos, Isabel; Sáez, Lara P.; Cabello, Purificación; Roldán, María Dolores; Moreno-Vivián, Conrado (Elsevier, 2015)tPseudomonas pseudoalcaligenes CECT5344 is an alkaliphilic bacterium that can use cyanide as nitrogensource for growth, becoming a suitable candidate to be applied in biological treatment of cyanide-containing wastewaters. ... -
Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
Olaya-Abril, Alfonso; Hidalgo-Carrillo, J.; Luque-Almagro, Víctor Manuel; Fuentes-Almagro, Carlos; Urbano, Francisco José; Moreno-Vivián, Conrado; Richardson, David J.; Roldán Alfonso, María Dolores (Nature, 2021)Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which ... -
Exploring anaerobic environments for cyanide and cyano-derivatives microbial degradation
Luque-Almagro, Víctor Manuel; Cabello, Purificación; Sáez, Lara P.; Olaya-Abril, Alfonso; Moreno-Vivián, Conrado; Roldán, María Dolores (SpringerLink, 2018)Cyanide is one of the most toxic chemicals for living organisms described so far. Its toxicity is mainly based on the high affinity that cyanide presents toward metals, provoking inhibition of essential metalloenzymes. ... -
Exploring the denitrification proteome of paracoccus denitrificans PD1222
Olaya-Abril, Alfonso; Hidalgo-Carrillo, J.; Luque-Almagro, Víctor Manuel; Fuentes-Almagro, Carlos; Urbano, Francisco José; Moreno-Vivián, Conrado; Richardson, David J.; Roldán, María Dolores (Frontiers, 2018)Denitrification is a respiratory process that produces nitrous oxide as an intermediate, which may escape to the atmosphere before its reduction to dinitrogen through the nitrous oxide reductase NosZ. In this work, the ... -
Genomic Insights into Cyanide Biodegradation in the Pseudomonas Genus
Sáez, Lara P.; Rodríguez Caballero, Gema; Olaya-Abril, Alfonso; Cabello, Purificación; Moreno-Vivián, Conrado; Roldán, María Dolores; Luque-Almagro, Víctor Manuel (MDPI, 2024)Molecular studies about cyanide biodegradation have been mainly focused on the hydrolytic pathways catalyzed by the cyanide dihydratase CynD or the nitrilase NitC. In some Pseudomonas strains, the assimilation of cyanide ... -
Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003
Biełło, Karolina A.; Lucena, Carlos; López Tenllado, Francisco Javier; Hidalgo-Carrillo, J.; Rodríguez Caballero, Gema; Cabello, Purificación; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Moreno-Vivián, Conrado; Olaya-Abril, Alfonso (Frontiers, 2023)Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield and quality. N and P chemical fertilizers are widely used in current agriculture, causing ... -
Nitrogen Oxyanion-dependent Dissociation of a Two-component Complex That Regulates Bacterial Nitrate Assimilation
Luque-Almagro, Víctor Manuel; Lyall, Verity J.; Ferguson, Stuart J.; Richardson, David J.; Gates, Andrew J.; Roldán, María Dolores (American Society for Biochemistry and Molecular Biology, 2013)Background: Nitrogen oxyanion-responsive two-component regulators that control assimilatory nitrate metabolism in heterotrophic bacteria are poorly characterized. Results: The nasT and nasS genes encode a regulatory ... -
Poly(3-hydroxybutyrate) hyperproduction by a global nitrogen regulator NtrB mutant strain of Paracoccus denitrificans PD1222
Olaya-Abril, Alfonso; Luque-Almagro, Víctor Manuel; Manso, Isabel; Gates, Andrew J.; Moreno-Vivián, Conrado; Richardson, David J.; Roldán, María Dolores (Oxford Academic, 2017)Paracoccus denitrificans PD1222 accumulates short-length polyhydroxyalkanoates, poly(3-hydroxybutyrate), under nitrogen-deficient conditions. Polyhydroxybutyrate metabolism requires the 3-ketoacyl-CoA thiolase PhaA, the ... -
Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344
Biełło, Karolina A.; Cabello, Purificación; Rodríguez Caballero, Gema; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Olaya-Abril, Alfonso; Moreno-Vivián, Conrado (MDPI, 2023)Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the ... -
Pseudomonas pseudoalcaligenes CECT5344, a cyanide‑degrading bacterium with by‑product (polyhydroxyalkanoates) formation capacity
Manso Cobos, Isabel; Ibáñez García, María Isabel; Peña Moreno, Fernando de la; Sáez Melero, Lara Paloma; Luque-Almagro, Víctor Manuel; Castillo Rodríguez, Francisco; Roldán Ruiz, María Dolores; Prieto Jiménez, María Auxiliadora; Moreno-Vivián, Conrado (BioMed Central, 2015)Background: Cyanide is one of the most toxic chemicals produced by anthropogenic activities like mining and jewelry industries, which generate wastewater residues with high concentrations of this compound. Pseudomonas ... -
Quantitative proteomic analysis of cyanide and mercury detoxification by pseudomonas pseudoalcaligenes CECT 5344
Biełło, Karolina A.; Olaya-Abril, Alfonso; Cabello, Purificación; Rodríguez Caballero, Gema; Sáez, Lara P.; Moreno-Vivián, Conrado; Luque-Almagro, Víctor Manuel; Roldán, María Dolores (American Society for Microbiology, 2023)The cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT 5344 uses cyanide and different metal-cyanide complexes as the sole nitrogen source. Under cyanotrophic conditions, this strain was able to grow with up ... -
Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-contain ing wastewaters using Liquid Chromatography- Mass Spectrometry/Mass Spectrometry (LC- MS/MS)
Ibáñez, María Isabel; Cabello, Purificación; Luque-Almagro, Víctor Manuel; Sáez, Lara P.; Olaya-Abril, Alfonso; Sánchez de Medina, Verónica; Luque de Castro, M.D.; Moreno-Vivián, Conrado; Roldán, María Dolores (PLOS, 2017)Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas ps ... -
The NtrYX Two-Component System of Paracoccus denitrificans Is Required for the Maintenance of Cellular Iron Homeostasis and for a Complete Denitrification under Iron-Limited Conditions
Olaya-Abril, Alfonso; Luque-Almagro, Víctor Manuel; Hidalgo-Carrillo, J.; Chicano-Gálvez, Eduardo; Urbano, Francisco José; Moreno-Vivián, Conrado; Richardson, David J.; Roldán, María Dolores (MDPI, 2022)Denitrification consists of the sequential reduction of nitrate to nitrite, nitric oxide, nitrous oxide, and dinitrogen. Nitrous oxide escapes to the atmosphere, depending on copper availability and other environmental ...