Browsing Departamento de Bioquímica y Biología Molecular by Issue Date
Now showing items 1-20 of 423
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Contaminación de pozos de la campiña de Córdoba. II. Estudio de la posible relación entre la contaminación por E. Coli y la concentración de algunos iones (cloruros, nitritos, y amoniaco) en el agua
(Universidad de Córdoba, Servicio de Publicaciones, 1979)The possible relation between chlorure, nitrite and ammonia ionic concentrations and qualitative-quantitative contamination by L. coli in water of wells located in the Campiña of Cordoba has been studied. No significative ... -
Mouse-Liver Glutathione Reductase: Inactivation by NADPH sf Two Allelic Variants
(CSIC, 1981)Mouse-liver glutathione reductase has been purified to homogeneity from strain SWR/J by ammonium sulfate precipitation (40-80 %) and two additional steps of affinity chromatography in ATPR-Sepharose and 2', 5'-ADP-Sepharose ... -
Biotecnología Agrícola
(Universidad de Córdoba, Servicio de Publicaciones, 1992) -
Direct Determination of Glutathione Reductase in Cells Cultured in Microtitre Plates as a Biomarker for Oxidative Stress
(Fund for the Replacement of Animals in Medical Experiments, 1995)A new method was developed for the direct determination of glutathione reductase (GOR) activity in Vero cells cultured in microtitre plates, avoiding cell-free extract preparation. The cells in each well were washed twice ... -
Direct Determination of Glutathione S-transferase and Glucose-6-phosphate Dehydrogenase Activities in Cells Cultured in Microtitre Plates as Biomarkers for Oxidative Stress
(Fund for the Replacement of Animals in Medical Experiments, 1998)The enzymes glutathione S,-transferase (GST) and glucose-6-phosphate dehydrogenase (G-6PDH) are implicated in the defence against oxidative stress. GST is mainly involved in the conjugation of electrophilic compounds ... -
Propuesta de metodología para la localización de microsatélites en el genoma equino
(Universidad de Córdoba, Servicio de Publicaciones, 1998)We present a relatively simple methodology for the detection of microsatellite sequences in partial genetic libraries in plasmids, consisting mainly in a new DNA extraction protocol and the use of a probe (TG)7T to locate ... -
Hacia un uso racional de los herbicidas
(Eumedia, 1999) -
Amplification and cloning of cDNAS of cytochrome P4501A1 and metallothionein genes from Sparus aurata Linnaeus, 1758 and Liza aurata (Risso, 1810) by Race-PCR
(Instituto Español de Oceanografía, 1999)The biotransformation of xenobiotics found in marine ecosystems is catalysed by inducible systems, a property that makes them useful as early-warning biomarkers of environmental pollution. We have focused our study on ... -
Nitrate is reduced by heterotrophic bacteria but not transferred to Prochlorococcus in non-axenic cultures
(Oxford Academic, 2002)The ability to assimilate nitrate in non-axenic isolates of Prochlorococcus spp. was addressed in this work, particularly in three low- irradiance adapted strains originating from ocean depths with measurable nitrate ... -
How bacteria get energy from hydrogen: a genetic analysis of periplasmic hydrogen oxidation in Escherichia coli.
(Elsevier, 2002)Dihydrogen oxidation is an important feature of bacterial energy conservation. In Escherichia coli hydrogen oxidation (‘uptake’) is catalysed by membrane-bound [NiFe] hydrogenase-1 and [NiFe] hydrogenase-2. The bulk of ... -
Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins
(Federation of European Biochemical Societies, 2003)The Escherichia coli twin-arginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with ... -
Coordinating assembly and export of complex bacterial proteins EMBO J. 76:409-406
(EMBO, 2004)The Escherichia coli twin-arginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with ... -
Desarrollo de estrategias moleculares para el estudio de la asimilación de nitrato en algas
(Universidad de Córdoba, Servicio de Publicaciones, 2005)En este trabajo se han desarrollado diferentes estrategias moleculares para el estudio de la ruta de asimilación de nitrato utilizando como organismo modelo el alga verde Chlamydomonas reinhardtii. Conocer los elementos ... -
Accesibilidad a Internet: requisitos para la calidad en la docencia e investigación
(Universidad de Córdoba, Vicerrectorado de Innovación y Calidad Docente, 2005)A good Internet platform for teaching and research must be accessible, independently of the computer platform used, language and other peculiarities (personal or external). This work analyzes the current limitations of ... -
Caracterización de un ADNc de fresas específico de frutos maduros que presenta homología con péptido metionima sulfóxido reductasas
(Universidad de Córdoba, Servicio de Publicaciones, 2005)Se ha clonado y caracterizado un cDNA de fresa que codifica un Péptido metionina Sulfóxido Reductasa (PMSR) de fresa. Este gen se expresa única y exclusivamente en los frutos maduros de fresa. La construcción de una proteína ... -
Identificación y trazabilidad del cerdo ibérico y productos derivados mediante técnicas genómicas
(Federación Española de Asociaciones de Ganado Selecto, 2005) -
Common principles in the biosynthesis of diverse enzymes
(Portland Press, 2005)A subset of bacterial periplasmic enzymes are transported from the cytoplasm by the twin-arginine transport apparatus. Such proteins contain distinctive N-terminal signal peptides containing a conserved SRRXFLK 'twin-arginine' ... -
Una visión actual de la infección del cultivo del girasol por roya
(Eumedia, 2006)En el presente artículo se describe de forma general y actual la enfermedad de roya del girasol, causada por el hongo Puccina helianthi, profundizando en la biología de la interacción, estrategias de control y riesgos ... -
Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms
(Annual Reviews, 2006)The photobiological production of H2 gas, using water as the only electron donor, is a property of two types of photosynthetic microorganisms: green algae and cyanobacteria. In these organisms, photosynthetic water splitting ... -
Glutamine synthetase degradation is controlled by oxidative proteolysis in the marine cyanobacterium Prochlorococcus marinus strain PCC 9511
(Elsevier, 2006)Prochlorococcus is one of the most important primary producers on Earth; its unusual features and ecological importance have made it a model organism, but nutrient assimilation has received little attention. Glutamine ...